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1:
buffer A:
date: 2009-06-06
pH: 8
contents:
- 100mM Tris
- 50mM EDTA
- 100mM NaCl
extraction buffer:
notes: SATA'S RESEARCH - DO NOT USE #'
pH: 8
date: 2009-02-20
contents:
- 100mM Tris-HCl
- 50mM EDTA
- 1% SDS
- 1% PVP
buffer A:
date: 2009-02-16
pH: 8
notes: SATA's RESEARCH - DO NOT USE #'
volume: 60 mL
extraction buffer:
date: 2009-06-06
pH: 8
contents:
- 100mM Tris-HCl
- 50mM EDTA
- 1% SDS
- 1% PVP
%Sarkosyl:
date: 2009-02-11
contents:
- 0.3g/30mL
NaCL:
date: 2009-02-26
contents:
- 5M NaCl
volume: 100mL
ethanol:
date: 2009-02-11
contents:
- ethanol
volume: 200mL
SDS:
date: 2007-11-30
contents:
- 5% SDS
notes: EH.
volume: 200 mL
NaOH:
date: 2007-06-18
contents:
- 0.1% NaOH
person: Kafi J.
volume: 1000mL
SSC:
date: 2009-03-27
volume: full
contents:
- 5x SSC
notes:
- L. R.
- L. B.
TES:
pH: 8
date: 2009-02-20
person:
- JT
- XY
contents:
- 50mM Tris
- 5mM EDTA
- 50mM MNaCl
volume: 50mL
acetone:
volume: 50mL
contents:
- acetone
SOB medium:
volume: 400mL
date: 2009-03-12
person: LB
contents:
- SOB medium
2:
neutralization solution:
date: 2007-11-13
person: RG
pH: 7.5
volume: 1000mL
untitled:
date: 2009-04-06
person: EC
contents:
- .1% SDS
- .1% SSC
volume: 500mL
HEPES:
contents:
- .1M HEPES
volume: 110mL
EDTA:
contents:
- 0.5M EDTA
pH: 8
volume:
- 100mL
- 50mL
date: 2007-01-19
person: RG
notes:
- BIO 331L Monday
- RNA only
denaturization solution:
date: 2008-10-26
person: KP
volume: 250mL
SSC:
contents:
- 0.5x SSC
- 0.1% SDS
date: 2008-11-04
person: KP
gel running buffer:
contents:
- 10x 614 oxal gel running buffer
date: 2009-03-27
person: EG
notes: RNA only!
volume: 250mL
TRIS:
contents:
- 1M Tris x2
pH: 7.6
notes:
- 5D
- 5P
date: 2009-02-09
volume: 150mL
SSC:
contents:
- 2x SSC
- .1% SDS
person: EC
date: 2009-04-06
volume: 800mL
DEPC:
contents:
- DEPC
- H2O
date: 2009-06-09
person: LB
volume: 200mL
NaCL:
contents:
- 3M NaCl
date: 2005-09-13
notes: C1RM
volume: 400mL
CTAB precipitation buffer:
contents:
#- CTAB precipitation buffer
- 1% CTAB
- 50mM Tris
- 10mM EDTA
date: 2007-05-18
person: MJ
volume: 200mL
NaOH:
contents:
- 0.4M NaOH
date: 2009-03-27
person: EC
volume: full
HCl:
contents:
- 250mM HCl
date: 2008-10-27
person: KP
volume: 450mL
3:
acetate:
contents:
- 3M Na acetate
pH: 5.2
date: 2007-04-13
checked: 2009-03-26
person:
- RR
- EC
notes: checked by EC 2009-03-26 for BIO 331L
volume: 200mL
DNA extraction buffer:
notes: steralized on 2008-04-21
date: 2008-04-16
volume:
- 100mL #start volume
- 50mL #current volume
person: IA
TE buffer:
notes: BIO 331L Monday
date: 2007-02-08
person: KH
contents:
- 10mM Tris-Cl
- 0.1mN EDTA
pH: 8
volume: 175mL
CaCl2:
date: 2009-06-24
person: LB
contents:
- CaCl2
volume: 450mL
TE buffer:
pH: 8
person: RL
date: 2007-11-28
volume: 850mL
transfer buffer:
notes: for SDS proteins
person: LB
date: 2009-04-15
volume: 400mL
DI water:
notes: autoclaved
person: LB
volume: 700mL
TRIS:
contents:
- 1M TRIS
pH: 8
date: 2005-10-13
notes: checked by RL (2008-05-09)
volume: 500mL
5x DNA annealing:
contents:
- 350mN TRIS
- 50mM MgCl2
- 50mM KCL
date: 2009-02-09
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