1: buffer A: date: 2009-06-06 pH: 8 contents: - 100mM Tris - 50mM EDTA - 100mM NaCl extraction buffer: notes: SATA'S RESEARCH - DO NOT USE #' pH: 8 date: 2009-02-20 contents: - 100mM Tris-HCl - 50mM EDTA - 1% SDS - 1% PVP buffer A: date: 2009-02-16 pH: 8 notes: SATA's RESEARCH - DO NOT USE #' volume: 60 mL extraction buffer: date: 2009-06-06 pH: 8 contents: - 100mM Tris-HCl - 50mM EDTA - 1% SDS - 1% PVP %Sarkosyl: date: 2009-02-11 contents: - 0.3g/30mL NaCL: date: 2009-02-26 contents: - 5M NaCl volume: 100mL ethanol: date: 2009-02-11 contents: - ethanol volume: 200mL SDS: date: 2007-11-30 contents: - 5% SDS notes: EH. volume: 200 mL NaOH: date: 2007-06-18 contents: - 0.1% NaOH person: Kafi J. volume: 1000mL SSC: date: 2009-03-27 volume: full contents: - 5x SSC notes: - L. R. - L. B. TES: pH: 8 date: 2009-02-20 person: - JT - XY contents: - 50mM Tris - 5mM EDTA - 50mM MNaCl volume: 50mL acetone: volume: 50mL contents: - acetone SOB medium: volume: 400mL date: 2009-03-12 person: LB contents: - SOB medium 2: neutralization solution: date: 2007-11-13 person: RG pH: 7.5 volume: 1000mL untitled: date: 2009-04-06 person: EC contents: - .1% SDS - .1% SSC volume: 500mL HEPES: contents: - .1M HEPES volume: 110mL EDTA: contents: - 0.5M EDTA pH: 8 volume: - 100mL - 50mL date: 2007-01-19 person: RG notes: - BIO 331L Monday - RNA only denaturization solution: date: 2008-10-26 person: KP volume: 250mL SSC: contents: - 0.5x SSC - 0.1% SDS date: 2008-11-04 person: KP gel running buffer: contents: - 10x 614 oxal gel running buffer date: 2009-03-27 person: EG notes: RNA only! volume: 250mL TRIS: contents: - 1M Tris x2 pH: 7.6 notes: - 5D - 5P date: 2009-02-09 volume: 150mL SSC: contents: - 2x SSC - .1% SDS person: EC date: 2009-04-06 volume: 800mL DEPC: contents: - DEPC - H2O date: 2009-06-09 person: LB volume: 200mL NaCL: contents: - 3M NaCl date: 2005-09-13 notes: C1RM volume: 400mL CTAB precipitation buffer: contents: #- CTAB precipitation buffer - 1% CTAB - 50mM Tris - 10mM EDTA date: 2007-05-18 person: MJ volume: 200mL NaOH: contents: - 0.4M NaOH date: 2009-03-27 person: EC volume: full HCl: contents: - 250mM HCl date: 2008-10-27 person: KP volume: 450mL 3: acetate: contents: - 3M Na acetate pH: 5.2 date: 2007-04-13 checked: 2009-03-26 person: - RR - EC notes: checked by EC 2009-03-26 for BIO 331L volume: 200mL DNA extraction buffer: notes: steralized on 2008-04-21 date: 2008-04-16 volume: - 100mL #start volume - 50mL #current volume person: IA TE buffer: notes: BIO 331L Monday date: 2007-02-08 person: KH contents: - 10mM Tris-Cl - 0.1mN EDTA pH: 8 volume: 175mL CaCl2: date: 2009-06-24 person: LB contents: - CaCl2 volume: 450mL TE buffer: pH: 8 person: RL date: 2007-11-28 volume: 850mL transfer buffer: notes: for SDS proteins person: LB date: 2009-04-15 volume: 400mL DI water: notes: autoclaved person: LB volume: 700mL TRIS: contents: - 1M TRIS pH: 8 date: 2005-10-13 notes: checked by RL (2008-05-09) volume: 500mL 5x DNA annealing: contents: - 350mN TRIS - 50mM MgCl2 - 50mM KCL date: 2009-02-09