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author: bryan bishop
license: GPL 2.0+
urls:
- http://groups.google.com/group/cryonics-europe/browse_thread/thread/d2a3e12a87308338?hl=en
- http://cryoeuro.eu/
- http://eugen.leitl.org/
name: basic CPA perfusion capability for Germany
equipment:
- graduated cylinders
- beakers
- pH standards (powdered)
- pH electrode
- portable ice bath
- http://eztube.com/
- heart lung resuscitator
- pneumatically driven CPR machine (thumper)
notes: do not use oxygen
- media bottles
notes: for high purity water or sterile perfusate storage
volume: 5 liters
number: 20
- stainless steel mixing tool
- cordless electric drill
- Jiffy mixer
- submersible water pump
- notes: to chill heat exchanger
- diffuser (garden hose type screw fitting)
- flexible rubber tubing (perfusate/silastic)
- polyethylene tanks
- arterial cannula, custom stainless steel femoral or carotid cannula, 2 each
- superscissors
- thermometers
- aquarium air pump
- air compressor
- anticoagulant
options:
- heparin
- neuropretectants
options:
- vitamin E
- melatonin
- curcumin
- NAC
- dry ice
offsite:
- liquid nitrogen storage units
stabilization protocol:
- Saline solution to dissoleve the heparin (2500 units of heparin per cc)
- or:
potassium chloride:
quantity: 1mEq/kg
sodium pentobarbital:
quantity: 30mg
- deferoxamine HCl:
quantity: 2g
- Epinephrine (0,2mg/kg followed by 0,1mg/kg every 10 min. until temp. is 25°C)
- or:
nimodipine:
notes: 10 micrograms/kg IV push + continuous IV infusion at a rate of 60 micrograms/Kg/h until esophageal temp is 30 deg C
diltiazem HCl:
quantity: 300 micrograms/Kg
- sodium acetate:
quantity: 120mg/kg
- E-Celle (micellized d-alpha-tocopherol acetate) (80 IU/Kg + continious infusion at a rate of 10 IU/Kg/h)
- ascorbic acid:
quantity: 125mg/kg
- Tromethamine (250mg/Kg; 200 cc 0,3M fast + infusion 30 drops/sec.)
- heparin:
quantity: 420 IU/kg
- chlorpromazine HCl:
quantity: 3mg/kg
- methlyprednisolone HCl:
quantity: 1g
- mannitol:
quantity: 2g/kg
- or:
metubine iodide:
quantity: 0.07 mg/Kg
succinylchloride:
quantity: 0.8 mg/Kg
#- Gentamicin sulphate (1 mg/Kg) + Bactrim (160 mg) + sulfamethoxazole (800 mg) or erythromycin (1 g adults, 500 mg children under 12)
- and:
gentamicin sulphate:
quantity: 1 mg/Kg
bactrim:
quantity: 160mg
or:
sulfamethoxazole:
quantity: 800 mg
erythromycin:
quantity: 1g
under 12 years of age: 500mg
- dextran-40 in normal saline:
quantity: 250..500cc
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