author: bryan bishop
license: GPL 2.0+
urls:
	- http://groups.google.com/group/cryonics-europe/browse_thread/thread/d2a3e12a87308338?hl=en
	- http://cryoeuro.eu/
	- http://eugen.leitl.org/
name: basic CPA perfusion capability for Germany

equipment:
	- graduated cylinders
	- beakers
	- pH standards (powdered)
	- pH electrode
	- portable ice bath
		- http://eztube.com/
	- heart lung resuscitator
	- pneumatically driven CPR machine (thumper)
        notes: do not use oxygen
	- media bottles
		notes: for high purity water or sterile perfusate storage
		volume: 5 liters
		number: 20
	- stainless steel mixing tool
	- cordless electric drill
	- Jiffy mixer
	- submersible water pump
		- notes: to chill heat exchanger
	- diffuser (garden hose type screw fitting)
	- flexible rubber tubing (perfusate/silastic)
	- polyethylene tanks
	- arterial cannula, custom stainless steel femoral or carotid cannula, 2 each
	- superscissors
	- thermometers
	- aquarium air pump
	- air compressor
    - anticoagulant
        options:
            - heparin
    - neuropretectants
        options:
            - vitamin E
            - melatonin
            - curcumin
            - NAC
    - dry ice

offsite:
    - liquid nitrogen storage units

stabilization protocol:
    - Saline solution to dissoleve the heparin (2500 units of heparin per cc)
    - or:
        potassium chloride:
            quantity: 1mEq/kg
        sodium pentobarbital:
            quantity: 30mg
    - deferoxamine HCl:
        quantity: 2g
    - Epinephrine (0,2mg/kg followed by 0,1mg/kg every 10 min. until temp. is 25°C)
    - or:
        nimodipine:
            notes: 10 micrograms/kg IV push + continuous IV infusion at a rate of 60 micrograms/Kg/h until esophageal temp is 30 deg C
        diltiazem HCl:
            quantity: 300 micrograms/Kg
    - sodium acetate:
        quantity: 120mg/kg
    - E-Celle (micellized d-alpha-tocopherol acetate) (80 IU/Kg + continious infusion at a rate of 10 IU/Kg/h)
    - ascorbic acid:
        quantity: 125mg/kg
    - Tromethamine (250mg/Kg; 200 cc 0,3M fast + infusion 30 drops/sec.)
    - heparin:
        quantity: 420 IU/kg
    - chlorpromazine HCl:
        quantity: 3mg/kg
    - methlyprednisolone HCl:
        quantity: 1g
    - mannitol:
        quantity: 2g/kg
    - or:
        metubine iodide:
            quantity: 0.07 mg/Kg
        succinylchloride:
            quantity: 0.8 mg/Kg
    #- Gentamicin sulphate (1 mg/Kg) + Bactrim (160 mg) + sulfamethoxazole (800 mg) or erythromycin (1 g adults, 500 mg children under 12)
    - and:
        gentamicin sulphate:
            quantity: 1 mg/Kg
        bactrim:
            quantity: 160mg
        or:
            sulfamethoxazole:
                quantity: 800 mg
            erythromycin:
                quantity: 1g
                under 12 years of age: 500mg
    - dextran-40 in normal saline:
        quantity: 250..500cc