Microfluidic equipment relevant to homebrew biology, diybio, biohacking, etc.


  • DVD mount, run in DVD player
  • food blender with custom chuck to hold slide


Sonoporation is probably the most simple for microfluidic designs.


  • "Separation of lipids from blood utilizing ultrasonic standing waves in microfluidic channels"
  • sound:
    • "Continuous particle separation in a microfluidic channel via standing surface acoustic waves (SSAW)"
    • "Noninvasive acoustic cell trapping in a microfluidic perfusion system for online bioassays"
    • "Chip integrated strategies for acoustic separation and manipulation of cells and particles"
  • tangential flow filtration

DNA separation

  • pillar/post/peg array
    • Sometimes with an electric field
      • "A DNA prism for high-speed continuous fractionation of large DNA molecules"
      • "Separation of long DNA molecules by quartz nanopillar chips under a direct current electric field"
  • jagged channel edge (see "Size-dependent free solution DNA electrophoresis in structured microfluidic systems")
  • electrophoresis
    • "Electrophoretic separations on microfluidic chips"
    • capillary electrophoresis
    • capillary gel electrophoresis (CGE)
    • microchip-based capillary electrophoresis
    • pulsed-field gel electrophoresis (PFGE)
    • capillary zone electrophoresis
    • micelle electrokinetic chromatography (MEKC)
    • isoelectric focusing (IEF)
      • isoelectric focusing also works for protein separation "Concentration and separation of proteins in microfluidic channels on the basis of transverse IEF"


The efficiency of chromatography increases with column length. Electrophoresis is often used in microfluidics instead of chromatography because "glass and plastics cannot withstand the pressures that would be needed; it is difficult to pack the channels without voids there are mass transfer problems in the corners of microchannels; voids cause unacceptable band broadening".

  • hydrodynamic chromatography
  • size exclusion chromatography
  • "High-aspect-ratio, silicon oxide-enclosed pillar structures in microfluidic liquid chromatography"
  • "Liquid phase chromatography on microchips"
  • "Fabrication of high-quality microfluidic solid-phase chromatography columns"
  • "Application of monoliths for bioparticle isolation"


  • digital isoelectric fractionation (using separate pH-specific membranes based on isoelectric points of analytes)
  • field flow fractionation
  • nanochannels with step-size changes corresponding to lengths of particles to separate


yep... many methods.

DNA sequencing

not yet

DNA synthesis


cell culture



http://en.wikipedia.org/wiki/Pipette#Microfluidic_pipette (just kidding.. use a regular pipette?)


isn't this already microfluidic? or is it millifluidic?