RE: BIOLOGY: Mouse and Human Genome similarity

From: Robert J. Bradbury (bradbury@aeiveos.com)
Date: Sun Dec 08 2002 - 06:48:00 MST


On Sat, 7 Dec 2002, Barbara Lamar wrote:

> Robert, as I have time to search more thoroughly, I'll see what I can find
> on the Internet with respect to cloning from single cells.

This would be interesting. You may want to include PubMed searches to
see if anyone has attempted dealing with this. [PubMed may not however
include all of the agricultural databases, so expanded searches may be
required ultimately.]

> Meanwhile, you might want to look at botanical tissue
> culture techniques in general.

Thanks for the references.

> The success of various tissue culture techniques varies from
> species to species. In most cases, you're still using more
> than a single cell.

I suspected this would be the case (w.r.t. the success rate
and # of cells). But there does appear to be a fairly direct
correlation between cloning in plants and mammals. The only
difference to me seems to involve the process of nuclear transfer.
But the fundamental question of whether the nucleus contains
a "viable" genome would seem to be the same in both cases.

It is a fundamental question that needs to be answered:
"What fraction of an adult entities cells are incapable
of regenerating a copy of itself?"

This has some implications with respect to cryonics because
the regeneration of a body (for head-only suspendees) may
not be quite so easy as people might imagine.

Robert



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