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Jan 2008</a></div></td></tr><tr><td width="145" valign="top" class="sidebar-cell"><div class="sidebar-article-navigation-box"><div class="sidefm-pmccurrent-item"><a class="sidefm-pmclink" href="javascript:return(false);"><span class="sidebar-menu-square-image-holder"><img src="corehtml/pmc/pmcgifs/square.gif" alt=">" style="border-style: none;"></span>Abstract</a></div><div class="sidefm-pmclink-item"><a href="articlerender.fcgi?artid=2151964" class="sidefm-pmclink">Full Text</a></div><div class="sidefm-pmclink-item"><a href="picrender.fcgi?artid=2151964&blobtype=pdf" class="sidefm-pmclink">PDF (458K)</a></div><div class="sidefm-pmclink-item"><a href="tocrender.fcgi?iid=157542" class="sidefm-pmclink">Contents</a></div><div class="sidefm-pmclink-item"><a href="tocrender.fcgi?journal=516&action=archive" class="sidefm-pmclink">Archive</a></div></div><div class="sidebar-pubmed-box"><div class="sidebar-pubmed-links-to-box"><form name="pubmedLinks" onsubmit="return pubMedDbLinkSubmit(this)"><div class="sidefm-pmsubhead"><label for="pubmedOption">Related material:</label></div><select name="pubmedOption" id="pubmedOption" class="sidefm-pmart" onchange="return pubMedDbLinkSubmit(this)"><option value="redirect3.cgi?&&auth=0B3ARgXMFgSotYIMMUT8XGMo3E6_vM1T3YMUU35Dz&reftype=abs&refto=entrez&reffrom=sidebar&article-id=2151964&issue-id=157542&journal-id=516&FROM=Article|Navigation&TO=Entrez|PubMed|Record&rendering-type=normal&&http://www.ncbi.nlm.nih.gov/pubmed/19002842">PubMed record</option><option selected value="redirect3.cgi?&&auth=0tiGaUK7JuRvvxxMu9QTzoWFK44F6vNPi4h0loQsQ&reftype=relart&refto=entrez&reffrom=sidebar&article-id=2151964&issue-id=157542&journal-id=516&FROM=Article|Navigation&TO=Entrez|PubMed|Related%20Records&rendering-type=normal&&http://www.ncbi.nlm.nih.gov/sites/entrez?db=PubMed&cmd=Link&dbFrom=PubMed&from_uid=19002842">PubMed related arts</option><option value="redirect3.cgi?&&auth=0TUNxxbXCeEx4LO5ZFOpR92xhW1-Gc_Cj2jA0L5Bj&reftype=linkout&refto=entrez&reffrom=sidebar&article-id=2151964&issue-id=157542&journal-id=516&FROM=Article|Navigation&TO=Entrez|PubMed|Linkout&rendering-type=normal&&http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowLinkOut&TermToSearch=19002842">PubMed LinkOut</option></select><br><input type="image" class="sidefm-pmart" alt="GO" src="corehtml/pmc/pmcgifs/go.gif"></form></div><div class="sidebar-pubmed-author-box"><div class="sidefm-pmsubhead">PubMed articles by:</div><div class="sidefm-pmclink-item"><a href="redirect3.cgi?&&auth=0vow9PJ3mULm-NAxXQC97iBG1nIY-YYc81ncgpQck&reftype=authsrch&refto=entrez&reffrom=sidebar&article-id=2151964&issue-id=157542&journal-id=516&FROM=Article%7CNavigation&TO=Entrez%7CPubMed%7CAuthor%20Search&rendering-type=normal&&http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=PubMed&term=%20Pinto%2BRC%5Bauth%5D" class="sidefm-pmclink"> Pinto, R.</a></div><div class="sidefm-pmclink-item"><a href="redirect3.cgi?&&auth=0JKf8eVoUw6uUD9-ICm82l8J_lgyHhWWeMNEGakZK&reftype=authsrch&refto=entrez&reffrom=sidebar&article-id=2151964&issue-id=157542&journal-id=516&FROM=Article%7CNavigation&TO=Entrez%7CPubMed%7CAuthor%20Search&rendering-type=normal&&http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=PubMed&term=%20Medronho%2BRA%5Bauth%5D" class="sidefm-pmclink"> Medronho, R.</a></div><div class="sidefm-pmclink-item"><a href="redirect3.cgi?&&auth=0-YwLsMEoPiLLbasYtfLx068-XhlTSmT4Af32m556&reftype=authsrch&refto=entrez&reffrom=sidebar&article-id=2151964&issue-id=157542&journal-id=516&FROM=Article%7CNavigation&TO=Entrez%7CPubMed%7CAuthor%20Search&rendering-type=normal&&http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=PubMed&term=%20Castilho%2BLR%5Bauth%5D" class="sidefm-pmclink"> Castilho, L.</a></div></div></div></td><td rowspan="100" width="20px" height="150" background="corehtml/pmc/pmcgifs/wm-cytotech.gif"><div style="width:20px"></div></td><td valign="top" class="content-cell"><div class="front-matter-section"><table cellspacing="0" cellpadding="0" width="100%"><tr style="vertical-align: top"><td><div class="fm-citation"><div><span class="citation-abbreviation">Cytotechnology. </span><span class="citation-publication-date">2008 January; </span><span class="citation-volume">56</span><span class="citation-issue">(1)</span><span class="citation-flpages">: 57–67. </span></div><div><span class="fm-vol-iss-date">Published online 2007 November 14. </span><span class="fm-vol-iss-date"> </span><span class="fm-vol-iss-date">doi: 10.1007/s10616-007-9108-x.</span></div></div></td><td class="fm-citation-ids"><div class="fm-citation-pmcid"><span class="fm-citation-ids-label">PMCID: </span>PMC2151964</div></td></tr></table><div class="fm-copyright"><a class="int-reflink" href="about/copyright.html">Copyright</a> © Springer Science+Business Media B.V. 2007</div><div class="fm-title">Separation of CHO cells using hydrocyclones</div><div class="contrib-group fm-author">Rodrigo C.V. Pinto,<sup>1</sup> Ricardo A. Medronho,<sup><img src="corehtml/pmc/pmcgifs/corrauth.gif" alt="corresponding author"></sup><sup>2</sup> and Leda R. Castilho<sup>1</sup></div><div class="fm-affl"><sup>1</sup>Federal University of Rio de Janeiro (UFRJ), COPPE - Chemical Engineering Program, Caixa Postal 68502, CEP 21941-972 Rio de Janeiro, RJ Brazil</div><div class="fm-affl"><sup>2</sup>School of Chemistry, Chemical Engineering Department, Federal University of Rio de Janeiro (UFRJ), CT, Bloco E, CEP 21949-900 Rio de Janeiro, RJ Brazil</div><div class="fm-footnote"><span class="fm-affl">Ricardo A. Medronho, </span><span class="fm-affl">Phone: +55-21-25627635, Fax: +55-21-25627567, Email: <span class="e_id124637">medronho/at/eq.ufr.br</span><script type="text/javascript" language="JavaScript"><!--
try{initUnObscureEmail ("e_id124637", '<a class="ext-reflink" href="' + reverseAndReplaceString('rb.rfu.qe/ta/ohnordem:otliam', '/at/', '@') + '">' + reverseAndReplaceString('rb.rfu.qe/ta/ohnordem', '/at/','@') + '</a>')}catch(e){}
//--></script></span>.</div><div class="fm-footnote"><sup><img src="corehtml/pmc/pmcgifs/corrauth.gif" alt="corresponding author"></sup>Corresponding author.</div><div class="fm-footnote"></div><div class="fm-pubdate">Received April 3, 2007; Accepted October 20, 2007.</div></div></td></tr><tr valign="top"><td class="sidebar-cell" width="145"> </td><td class="content-cell"><div class="head1 section-title" style="text-transform: none;" id="id147885">Abstract</div><div class="section-content"><!--article-meta--><div id="id147886" class="p p-first-last">Hydrocyclones are simple and robust separation devices with no moving parts. In the past few years, their use in animal cell separation has been proposed. In this work, the use of different hydrocyclone configurations for Chinese hamster ovary (CHO) cell separation was investigated following an experimental design. It was shown that cell separation efficiencies for cultures of the wild-type CHO.K1 cell line and of a recombinant CHO cell line producing granulocyte-macrophage colony stimulating factor (GM-CSF) were kept above 97%. Low viability losses were observed, as measured by trypan blue exclusion and by determination of intracellular lactate dehydrogenase (LDH) released to the culture medium. Mathematical models were proposed to predict the flow rate, flow ratio and separation efficiency as a function of hydrocyclone geometry and pressure drop. When cells were monitored for any induction of apoptosis upon passage through the hydrocyclones, no increase in apoptotic cell concentration was observed within 48 h of hydrocycloning. Thus, based on the high separation efficiencies, the robustness of the equipment, and the absence of apoptosis induction, hydrocyclones seem to be specially suited for use as cell retention devices in long-term perfusion runs.</div><div class="p"><span class="kwd-label">Keywords: </span><span class="kwd-text">Animal cells, Cell separation, CHO cells, Hydrocyclones, Perfusion, Retention device</span></div></div></td></tr><tr><td></td><td class="content-cell"><div class="footer-section"><hr><div class="sans90" style="margin: 0em 1em 1em; text-align: center;">Articles from <span class="acknowledgment-journal-title">Cytotechnology</span> are provided here courtesy of <br><b>Springer Science+Business Media B.V.</b></div>
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