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# MAPseq and BARseq
## Reviews
* **Barcode-based connectomics (concept + limitations summary, 2024)** — short overview contrasting MAPseq, BRICseq, BARseq, and ConnectID, incl. tradeoffs for adding transcriptomes. [eLife](https://elifesciences.org/articles/85419?utm_source=chatgpt.com)
* **From MAPseq to BRICseq and beyond (2021)** — narrative review of barcoded projection mapping from the Zador lab lineage. [PMC](https://pmc.ncbi.nlm.nih.gov/articles/PMC8099946/?utm_source=chatgpt.com)
* **Neural circuit research with molecular barcodes (2025)** — broader survey placing MAPseq/BARseq among barcoded neurotech. [ScienceDirect](https://www.sciencedirect.com/science/article/pii/S0168010225000549?utm_source=chatgpt.com)
## MAPseq — essentials
* **MAPseq (Neuron, 2016)** — seminal method: infect neurons with a diverse RNA-barcode virus; bulk-sequence dissected target regions to read one-to-many projections at scale. (Open access.) [PMC](https://pmc.ncbi.nlm.nih.gov/articles/PMC6640135/?utm_source=chatgpt.com)
* **MAPseq (primary index / PubMed)** — canonical reference & metadata. [PubMed](https://pubmed.ncbi.nlm.nih.gov/27545715/?utm_source=chatgpt.com)
* **MAPseq2 (preprint, 2025)** — protocol update reporting \~3–4× higher barcode detection sensitivity and \~10× lower cost vs. original MAPseq. [ResearchGate](https://www.researchgate.net/publication/392975273_MAPseq2_a_sensitive_and_cost-effective_barcoded_connectomics_method?utm_source=chatgpt.com)
# See also
See [[in situ sequencing]] for more about BARseq, BARseq2, etc.
See [[lineage tracing]] for other cell tracing and cell lineage tracing techniques.
One interesting alternative is *compressive sensing* like "[Rapid learning of neural circuitry from holographic ensemble stimulation enabled by model-based compressed sensing](https://www.biorxiv.org/content/10.1101/2022.09.14.507926v3.abstract)" or this [ref](https://www.cell.com/patterns/fulltext/S2666-3899(23)00224-6).
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