gene-therapy.mdwn


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Here are some gene therapy delivery methods.

# naked DNA

# hydrodynamic injection

# viruses and viral gene therapy

* [CAV-2 - why a canine virus is a neurobiologist's best friend](http://easco.org/home/sites/default/files/files_rep/pdf/CAV2why%20a%20canine%20virus.pdf)

# electroporation

# gene gun

# sonoporation

# gas-filled micro-bubbles

# magnetofection and magnetic nanoparticles

* magnetic calcium phosphate nano-formulations

# carbon nanotubes

# quantum dots

* super paramagnetic iron oxide nanoparticles (SPIONS)

# lipoplexes

# dendrimers

# inorganic nanoparticles

* carbon nanotuubes
* magnetic nanoparticles (see also magnetofection)
* calcium phosphate nanoparticles
* gold nanoparticles
* quantum dots
* C60 fullerenes
* aminofullerenes
* silica nanoparticles

# gold nanoparticles

# cell-penetrating peptides

# polymerosomes

# liposomes

# polyplexes (DNA + plastic)

* polyion complex micelles (PICs)

# cationic polymers

* collagen
* albumin
* beta-casein
* zein

# cationic lipids

* dioleylpropyl trimethylammonium chloride (DOTMA)
* dioleoyl trimethylammonium propane (DOTAP)

# cationic emulsions

# solid lipid nanoparticles

# virosomes

# protofection

# natural competence

# Ligandal's nano-gobstopper method

(patent) [Nanoparticle-mediated gene delivery, genomic editing and ligand-targeted modification in various cell populations](https://patents.google.com/patent/CA2924535A1)

conjugation of PEI for DNA binding, histone tail peptide for nuclear localization, poly-glutamic acid, glutamic acid for stabilization and charge balancing or whatever, then you stick DNA to it, and silica coat the whole thing, then add even more cell-targeting stuff to the outside.

# other

* poly lactic-co-glycolic acid (PLGA)-based nanoparticles
* hybrid nanoparticles such as liposome-polycation-DNA (LPD) nanoparticles
* lipopolyplexes

# hplusroadmap logs

See <http://gnusha.org/logs/2017-01-09.log> for some preliminary stuff.