It seems likely that brain uploading will occur via in situ sequencing methods like fisseq combined with expansion microscopy. Also axonal tracing via lineage tracing techniques or other connectome data extraction. If we need even greater detail recovered from human brain, then possibly DNA nanoscopy could be used.
DNA ticker tape memory devices and intracellular protein ticker tape memory devices are also of high relevance to the question of brain uploading.
See fisseq for information about brain uploading.
See human brain reverse engineering for some other ideas.
Automated scalable segmentation of neurons from multispectral images
Probabilistic fluorescence-based synapse detection
Stochastic single-molecule dynamics of synaptic membrane protein domains
Quantifying mesoscale neuroanatomy using X-ray microtomography
The demise of the synapse as the locus of memory: A looming paradigm shift?
Connectome-seq: high-throughput mapping of neuronal connectivity at single-synapse resolution via barcode sequencing as described on the mapseq page.
Combinatorial protein barcodes enable self-correcting neuron tracing with nanoscale molecular context and E11-prism
Brain uploading companies
https://eon.systems/ is focusing on human brain emulation. Nectome is focused on human brain preservation in cryonics. E11 Bio is focused on synaptic barcoding and connectome DNA sequencing. There was also the startup venture (Brain Backups) doing microCT (high-resolution x-ray computed tomography) of gold aptamer conjugates for targeting synapses or receptors and then doing regular imaging.
Brain imaging
Deep brain fluorescence imaging with minimally invasive ultra-thin optical fibers
Automated scalable segmentation of neurons from multispectral images
Probabilistic fluorescence-based synapse detection
Quantifying mesoscale neuroanatomy using X-ray microtomography
Morphological error detection in 3d segmentations
Comparative prospects of imaging methods for whole-brain mammalian connectomics
Predicting functional neuroanatomical maps from fusing brain networks with genetic information (2016)
Super-resolution imaging of synaptic and extra-synaptic pools of AMPA receptors with different-sized fluorescent probes and https://x.com/biorxiv_neursci/status/813942699879497729
Multiplexed confocal and super-resolution fluorescence imaging of cytoskeletal and neuronal synapse proteins and https://x.com/eboyden3/status/836175796117065728
Rapid whole brain imaging of neural activities in freely behaving larval zebrafish (2017)
Whole-brain serial-section electron microscopy in larval zebrafish (2017)
A complete electron microscopy volume of the brain of adult drosophila melanogaster (2017)
Oblique light-sheet tomography: fast and high resolution volumetric imaging of mouse brains (2017)
In vivo magnetic recording of neuronal activity (2017)
Scalable volumetric imaging for ultrahigh-speed brain mapping at synaptic resolution (2017)
Deep image reconstruction from human brain activity (2017)
Freeze-frame imaging of synaptic activity using SynTagMA
Population imaging of neural activity in awake behaving mice in multiple brain regions and https://x.com/eboyden3/status/1121778597205827584
Enabling FIB-SEM systems for large volume connectomics and cell biology
Automatic whole cell organelle segmentation in volumetric electron microscopy
Synaptic labeling with engineered proteins (e.g. GRASP, mGRASP, SynTagMA, SynaptoTag): Highlight synaptic contacts between defined populations.
Combinatorial protein barcodes enable self-correcting neuron tracing with nanoscale molecular context and E11-prism
russell hanson has a technique for gold nanoparticle aptamer conjugates for microCT x-ray imaging of the distribution of neurotransmitter receptors throughout human brain matter as a non-destructive imaging technique.
See also brain uploading.
See also brain imaging.