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From Dynein achieves processive motion using both stochastic and coordinated stepping

  • Weihong Qiu1, 8
  • Nathan D Derr1, 2, 3, 8
  • Brian S Goodman1
  • Elizabeth Villa4, 5
  • David Wu5, 6
  • William Shih2, 3, 7
  • Samara L Reck-Peterson1
Journal name:
Nature Structural & Molecular Biology
Volume:
19,
Pages:
193–200
Year published:
(2012)
DOI:
doi:10.1038/nsmb.2205

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Figures

  1. Dynein structure and constructs used in this study.

    Figure 1

    Dynein structure and constructs used in this study.

    • Full size figure and legend
  2. Two-dimensional stepping analysis of GST–dynein homodimers.

    Figure 2

    Two-dimensional stepping analysis of GST–dynein homodimers.

    • Full size figure and legend
  3. DNA-based dynein heterodimers are functional and step similarly to protein-based dynein homodimers.

    Figure 3

    DNA-based dynein heterodimers are functional and step similarly to protein-based dynein homodimers.

    • Full size figure and legend
  4. Two-color tracking of dynein stepping.

    Figure 4

    Two-color tracking of dynein stepping.

    • Full size figure and legend
  5. Spatial arrangement of dynein motor domains during the two-head-bound state.

    Figure 5

    Spatial arrangement of dynein motor domains during the two-head-bound state.

    • Full size figure and legend
  6. Dynein steps are stochastic at short head-to-head spacing and coordinated as head-to-head spacing increases.

    Figure 6

    Dynein steps are stochastic at short head-to-head spacing and coordinated as head-to-head spacing increases.

    • Full size figure and legend

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Additional data

Entities in this article

  • Dynein heavy chain, cytoplasmic

    DYN1

    Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

    View:
    • In UniProt - opens in external window
    • In NCBI Gene - opens in external window
  • Glutathione S-transferase class-mu 26 kDa isozyme

    Schistosoma japonicum

    View:
    • In UniProt - opens in external window
  • Kinesin-1 heavy chain

    KIF5B

    Homo sapiens

    View:
    • In UniProt - opens in external window
    • In NCBI Gene - opens in external window
    Find antibodies in Antibodypedia - opens in external window
  • Myosin-Va

    MYO5A

    Homo sapiens

    View:
    • In UniProt - opens in external window
    • In NCBI Gene - opens in external window
    Find antibodies in Antibodypedia - opens in external window
  • Kinesin-like protein KIP3

    KIP3

    Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

    View:
    • In UniProt - opens in external window
    • In NCBI Gene - opens in external window
  • Myosin-VI

    MYO6

    Homo sapiens

    View:
    • In UniProt - opens in external window
    • In NCBI Gene - opens in external window
    Find antibodies in Antibodypedia - opens in external window
  • Nuclear distribution protein PAC1

    PAC1

    Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

    View:
    • In UniProt - opens in external window
    • In NCBI Gene - opens in external window
  • Nuclear distribution protein nudE homolog 1

    NDL1

    Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

    View:
    • In UniProt - opens in external window
    • In NCBI Gene - opens in external window
  • Orotidine 5'-phosphate decarboxylase

    KLLA0E22771g

    Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)

    View:
    • In UniProt - opens in external window
    • In NCBI Gene - opens in external window

Primary authors

  1. These authors contributed equally to this work.

    • Weihong Qiu &
    • Nathan D Derr

Affiliations

  1. Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA.

    • Weihong Qiu,
    • Nathan D Derr,
    • Brian S Goodman &
    • Samara L Reck-Peterson
  2. Dana Farber Cancer Institute, Boston, Massachusetts, USA.

    • Nathan D Derr &
    • William Shih
  3. Department of Biochemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA.

    • Nathan D Derr &
    • William Shih
  4. Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Martinsried, Germany.

    • Elizabeth Villa
  5. Physiology Course, Marine Biological Laboratory, Woods Hole, Massachusetts, USA.

    • Elizabeth Villa &
    • David Wu
  6. Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California, USA.

    • David Wu
  7. Wyss Institute, Harvard University, Boston, Massachusetts, USA.

    • William Shih

Contributions

W.Q. and N.D.D. contributed equally. W.Q., N.D.D., W.S. and S.L.R.-P. designed the experiments. W.Q., N.D.D. and B.S.G. conducted the experiments and analyzed the data. W.Q., N.D.D., B.S.G. and S.L.R.-P. wrote the paper. E.V. and D.W. wrote the two-dimensional particle tracking code.

Competing financial interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to:

  • Samara L Reck-Peterson

Author details

  • Weihong Qiu

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  • Elizabeth Villa

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  • David Wu

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  • Samara L Reck-Peterson

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Nature Structural & Molecular Biology
ISSN: 1545-9993
EISSN: 1545-9985
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