Authors: Valderrama, Maria-José; de Silo ´Niz, M. Isabel; Gonzalo, Pilar; Peinado, José M.

Source: Journal of Food Protection®, Volume 62, Number 2, February 1999 , pp. 189-193(5)

Publisher: International Association for Food Protection

Abstract:

A selective and differential solid medium, called Kluyveromyces Differential Medium (KDM), is described for the isolation of Kluyveromyces marxianus and K. lactis from dairy products. Its discriminative potential is based on the detection of the enzyme -galactosidase, in the absence of lactose. Of the more than 95 strains tested, including yeasts, bacteria, and filamentous fungus, only the strains of K. marxianus and K. lactis produced blue colonies on the medium due to the presence of X-Gal/IPTG. The bacterial strains were not able to grow in KDM. On this basis, the medium was very satisfactory when testing naturally or experimentally contaminated dairy food products. When quality assessment tests were performed, optimal values of productivity (growth and color) and selectivity were obtained for K. marxianus and K. lactis.

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Document Type: Research article

Affiliations: 1: Departamento de Microbiología, Facultad de Biología, Universidad Complutense de Madrid, 28040 Madrid, Spain

Publication date: 1999-02-01

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