Equipment: Cost (USD) Large centrifuge $9,000 Centrifuge rotors $15,000 Laminar flow hood $4,000 10L Shaking incubator $4,000 Large pressure cooker for autoclaving $300 Stirring hot plate + stir bars $500 Scales (3kg & 100g) $500 2L Erlenmeyer flasks (6+) $400 Centrifuge bottles (1L, 250mL, 50mL) $3000 1L & 250mL graduated cylinders $200 18.2 megaohm water dispenser $4000 Spectrometer $2000 pH meter $300 Gel electrophoresis apparatus & power supply $500 UV lamp $200 Vacuum pump $200 Macro- & micropipettors $400 1 liter bottles $200 Oven Dual-use Microwave Dual-use Refrigerator + freezer Dual-use Total: $50,000 Reagents (per 100mg of DNA): Cost (USD) Qiagen Gigaprep columns $1,000 Pipettes & pipette tips Negligible Filter papers* Negligible Tris powder* $100 EDTA powder* Negligible Sodium hydroxide pellets Negligible Potassium acetate* $50 Glacial acetic acid Negligible MOPS powder $100 Isopropanol $100 Triton X-100 Negligible Triton X-114* Negligible Ethanol $100 Hydrochloric acid Negligible NaCl powder $50 Tryptone powder* Negligible Yeast extract powder* Negligible Agar powder* Negligible Ethidium bromide solution* Negligible Ampicillin powder* Negligible RNAse A solution* $100 Dulbecco's PBS (recommend pre-made) Negligible Total: <$5,000 *=does not need to be laboratory-grade (i.e. will not interact with downstream plasmid requiring high-quality reagents)