Type I restriction enzyme with RecA protein promotes illegitimate recombination A new type of illegitimate recombination is dependent on restriction and homologous interaction. Site-directed alteration of genomic DNA by small-fragment homologous replacement Random-walk model of homologous recombination Discrete and continuous mathematical models of DNA branch migration Single molecule fluorescence analysis of branch migration of Holliday junctions: effect of DNA sequence Multiple cellular mechanisms prevent chromosomal rearrangements involving repetitive DNA Real-time observation of RecA filament dynamics with single monomer resolution Relative rates of homologous and nonhomologous recombination in transfected DNA Recombination between similar but not identical DNA sequences during yeast transformation occurs within short stretches of identity Minimum Length of Sequence Homology Required forin VivoCloning by Homologous Recombination in Yeast recA-independent and recA-dependent intramolecular plasmid recombination. Differential homology requirement and distance effect We show that recombination between directly repeated sequences in plasmids occurs via both recA-independent and recA-dependent mechanisms in Escherichia coli. They are differentially affected by two factors, the distance separating the homologies and the size of the homology. Recombination between tandem duplications up to 300 base-pairs shows virtually no recA dependence. Increasing the size of the duplications beyond 300 base-pairs gradually increases the recA dependence. Furthermore, insertion of a sizable DNA sequence in between the duplications, substantially increases the recA dependence. We conclude that increasing the distance separating the homologous regions preferentially inhibits the recA -independent recombination. On the other hand, shortening of the homology preferentially inhibits recA-dependent recombination. Consequently. recombination between short tandem duplications is totally recA -independent. Rapid cloning by homologous recombination in vivo A rapid method for recombination and site-specific mutagenesis by placing homologous ends on DNA using polymerase chain reaction Restriction-ligation-free (RLF) cloning: a high-throughput cloning method by in vivo homologous recombination of PCR products High-throughput cloning of Campylobacter jejuni ORFs by in vivo recombination in Escherichia coli RecA-independent recombination is efficient but limited by exonucleases FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts A simple and efficient seamless DNA cloning method using SLiCE from Escherichia coli laboratory strains and its application to SLiP site-directed mutagenesis AQUA cloning: A versatile and simple enzyme-free cloning approach Cloning should be simple: Escherichia coli DH5α-mediated assembly of multiple DNA fragments with short end homologies Optimal Cloning of PCR Fragments by Homologous Recombination in Escherichia coli Bacterial recombineering: genome engineering via phage-based homologous recombination Toward a multicolor chromosome bar code for the entire human karyotype by fluorescence in situ hybridization