Yeast Complete (YC) Media
100X YC (7 Dropout Amino Acid Liquid)
1.0 g arginine
0.5 g aspartic acid
0.5 g isoleucine
0.5 g phenylalanine
0.5 g proline
0.5 g serine
1.0 g threonine
0.5 g valine
in final volume of 100 ml
stir with low heat until into solution
filter sterilze
0.5 g aspartic acid
0.5 g isoleucine
0.5 g phenylalanine
0.5 g proline
0.5 g serine
1.0 g threonine
0.5 g valine
in final volume of 100 ml
stir with low heat until into solution
filter sterilze
10X YNB + tyrosine
17 g YNB (w/o amino acids and w/o ammonium sulfate)
50 g ammonium sulfate
0.5 g tyrosine
in a final volume of 1L
stir with low heat until into solution
filter sterilze
50 g ammonium sulfate
0.5 g tyrosine
in a final volume of 1L
stir with low heat until into solution
filter sterilze
Amino Acid Solutions (100X stocks, except URA=10X stock)
URA 1 mg/ml
ADE 1 mg/ml
LYS 10 mg/ml
MET 5 mg/ml
TRP 10 mg/ml
LEU 10 mg/ml
HIS 5 mg/ml
Autoclave to sterilize, except filter sterilize TRP
ADE 1 mg/ml
LYS 10 mg/ml
MET 5 mg/ml
TRP 10 mg/ml
LEU 10 mg/ml
HIS 5 mg/ml
Autoclave to sterilize, except filter sterilize TRP
Store all stocks at 4 deg C, except LEU (store at RT)
Example Recipe of Complete Plates
20 g of Agar in 630 ml distilled water in a 2L flask
Autoclave 20 minutes
Add the following:
Autoclave 20 minutes
Add the following:
| 100 ml | 20% sterile glucose |
| 100 ml | 10X YNB solution |
| 10 ml | 100X YC-7aa dropout solution |
| 100 ml | URA solution |
| 10 ml | ADE solution |
| 10 ml | LYS solution |
| 10 ml | MET solution |
| 10 ml | TRP solution |
| 10 ml | LEU solution |
| 10 ml | HIS solution |
FOA plates
20 g of Agar in 710 ml distilled water in a 2L flask
Autoclave 20 minutes
Add the following:
Autoclave 20 minutes
Add the following:
| 100 ml | 20% sterile glucose |
| 100 ml | 10X YNB solution |
| 10 ml | 100X YC-7aa dropout solution |
| 20 ml | URA solution |
| 10 ml | ADE solution |
| 10 ml | LYS solution |
| 10 ml | MET solution |
| 10 ml | TRP solution |
| 10 ml | LEU solution |
| 10 ml | HIS solution |
Let flask cool to 50 deg C, then add 1.0 g of 5-FOA.
