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Abstract for pGL3 Luciferase Reporter Vectors
The pGL3 Luciferase Reporter Vectors provide a basis for the quantitative
analysis of factors that potentially regulate mammalian gene expression. These
factors may be cis-acting, such as promoters and enhancers, or trans-acting,
such as various DNA-binding factors. The backbone of the pGL2 Luciferase
Reporter Vectors was redesigned for the pGL3 Vectors for increased expression,
and contains a modified coding region for firefly (Photinus pyralis)
luciferase that has been optimized for monitoring transcriptional activity in
transfected eukaryotic cells. The assay of this genetic reporter is rapid,
sensitive and quantitative. In addition, the Luciferase Reporter Vectors contain
numerous features aiding in the structural characterization of the putative
regulatory sequences under investigation.
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