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Abstract for Erase-a-Base® System
The Erase-a-Base® System is designed for the rapid construction
of plasmid or M13 subclones containing progressive unidirectional deletions of
any inserted DNA. The system is based on the procedure developed by Henikoff, in
which exonuclease III (Exo III) is used to specifically digest insert DNA from a
5´ protruding or blunt end restriction site. The adjacent sequencing primer
binding site is protected from digestion by a 4-base 3´ overhang restriction
site or by an alpha-phosphorothioate-filled end.
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