Abstract for Packagene^® Lambda DNA Packaging System

Part# TB005
Printed in USA. Revised 5/09.
Instructions for Use of Products K3152 & K3154.

The Packagene^® Lambda DNA Packaging System is derived from the unique one-strain host system (Rosenberg). The System is easy to use because it contains all the components and buffers needed to perform the packaging reaction in a single tube. Only the addition of substrate DNA is required. The packaging efficiencies of recombinant DNA using the single-strain Packagene^® System are as efficient as those using a two-strain system.

The Packagene^® Extract is produced from an E. coli C bacterial host. Libraries constructed using the Packagene^® System are not biased by EcoK restriction of genetic material because E. coli C lacks the K-12 DNA restriction system. The Positive Control DNA provided with the system, lambda cI857 Sam7, allows evaluation of Packagene^® System performance without depleting sample DNA.