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When isolating RNA, it helps to have a rough idea
of how much total or poly(A) RNA can be recovered from a given amount
of tissue or cells. It is also beneficial to know rough expression
levels for rare to moderately abundant transcripts when performing
Northern analysis or RPAs, e.g. in order to set up a meaningful standard
curve for absolute quantitation, or to know how long of an exposure
is reasonable. These numbers provide a point of reference against
which experimental procedures and results can be checked. Tables
1 and 2 provide general guidelines for estimating RNA yields from
a variety of cells and tissues. Remember that RNA yields from analogous
tissues in different organisms can also vary:
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| Cat# |
Product Name |
Size |
| AM12225 |
Thin-walled, Frosted Lid, RNase-free PCR Tubes |
1000 x 0.2 ml |
| AM12230 |
8-strip PCR Tubes & Caps, RNase-free, 0.2 ml |
125 strips (1000 tubes) |
| AM12250 |
Thin-walled, Dome Cap, RNase-free PCR Tubes |
1000 x 0.5 ml |
| AM12275 |
Thin-walled, Frosted Lid, RNase-free PCR Tubes |
1000 x 0.5 ml |
| AM1710 |
RETROscript® Kit |
40 rxns |
| AM1906 |
DNA-free™ |
50 rxns |
| AM1910 |
ToTALLY RNA™ Kit |
10 g tissue |
| AM1911 |
RNAqueous®-Midi Kit |
15 purifications |
| AM1912 |
RNAqueous® Kit |
50 purifications |
| AM1914 |
RNAqueous®-4PCR Kit |
30 rxns |
| AM1916 |
Poly(A)Purist™ Kit |
6 purifications |
| AM1920 |
RNAqueous®-96 Kit |
192 purifications |
| AM2050 |
SuperTaq™ Polymerase (Cloned) 5 U/µl |
50 U |
| AM2052 |
SuperTaq™ Polymerase (Cloned) 5 U/µl |
250 U |
| AM2054 |
SuperTaq™ Plus Polymerase (Cloned) 5 U/µl |
50 U |
| AM2056 |
SuperTaq™ Plus Polymerase (Cloned) 5 U/µl |
250 U |
| AM9738 |
TRI Reagent® |
100 ml |
| AM9890 |
DNAZap™ |
250 ml of each solution |
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