PROTOCOLS FOR RECOMBINANT DNA ISOLATION, CLONING, and SEQUENCING

             edited by

                Bruce A. Roe
                Judy S. Crabtree
		and Akbar S. Khan
		Department of Chemistry and Biochemistry
                The University of Oklahoma
                Norman, Oklahoma 73019

             e-mail:  broe@ou.edu
             phone:   405 325-4912
             fax:     405 325-7762
             August 5, 1995

A printed version of this protocol book can be obtained from: 


      "DNA Isolation and Sequencing" (Essential Techniques Series)
      by Bruce A. Roe, Judy S. Crabtree and Akbar S. Khan
      Published by John Wiley & Sons, List Price $49.95
      ISBN 0-471-97324-0
      QP625.N89R64  1996
      John Wiley & Sons
Introduction

This manual is a compilation of many of the everyday methods used in the average molecular biology laboratory, with emphasis on the techniques for large scale DNA sequencing protocols and DNA sequencing automation techniques. The manual has been written in a protocol format, with little theoretical discussion. For theory and additional information, users of this manual are referred back to the original literature, or to other textual manuals such as those published by Maniatis (1) et al. and Glover (2).

The following persons are acknowledged for contributing methods and suggestions during the assembly of this manual: Stephanie Chissoe, Sandy Clifton, Dennis Burian, Rick Wilson, Din-Pow Ma, James Wong, Leslie Johnston-Dow, Elaine Mardis, Zhili Wang, Kala Iyer, Steve Toth, Goughay Zhang, Hua Qin Pan and other members of the Roe laboratory, both past and present.

1. Sambrook, J., Fritsch, E.F., and Maniatis, T., in Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, NY, Vol. 1, 2, 3 (1989).

2. Glover, D.M. DNA Cloning Volume I: A Practical Approach. IRL Press, Oxford, 1985.

Table of Contents

Bruce A. Roe, Department of Chemistry and Biochemistry, The University of Oklahoma, Norman, Oklahoma 73019 broe@ou.edu