PCR Primer Design
 

Criteria for designing oligonucleotide primers.

  1. Sequences are highly conserved among prokaryotes.
  2. Segments of amplified fragment show significant variation.
  3. Fragment size between 350 and 600 bp is optimal for PCR and sequencing from both ends.
      Primer A - 28 nts - coding strand (~310 - ~340) 5’-CGGCCCAGACTCCTACGGGAGGCAGCA- 3’
       

      Primer B - 26 nts - non-coding strand (~770 - ~740) 
      5’-GCGTGGACTACCAGGGTATCTAATCC- 3’
       


 


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This page was created or last modified on 5/13/98 by Jeff Newman .
 
Assistant Professor Web page: http://lyco.lycoming.edu/~newman 
Department of Biology Email: newman@lycoming.edu
Lycoming College Phone: 717-321-4386
Williamsport PA 17701 Fax: 717-321-4073
© 1998  Jeffrey D. Newman