PCR Box Titration Calculator
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version 4 or higher required.
Experiment Title:
Investigator:
Date:
Columns
Rows
Reaction volume:
m
l
Fudge factor:
%
Titrate
Bottom
Top
Both
Reagent
Stock concentration
Final concentration (highest in titration)
buffer
X
X
dNTPs
m
M each
m
M each
MgCl
2
mM
mM
5' primer
m
M
m
M
3' primer
m
M
m
M
red sucrose
X
X
template
X
X
polymerase
U/
m
l
U/
m
l
additive
X
X
H
2
O