Applications, Techniques, and Protocols
Immunolabeling
Immunogold Labeling with BioSite Colloidal
Gold Reagents
BioSite Cationic Colloidal Gold
Immunogold Labelling in Scanning Electron
Microscopy
Microwave Applications, Techniques and Procedures
Special Stains Using the Microwave
Joyce Moore of Jefferson Regional Medical Center, Pine Bluff AR
Microwave Processing Techniques for Microscopy
Energy Beam Sciences, Inc.
References for Microwave Polymerization of
EM Resins
Microwave Antigen Retrieval Technique
Shi, Shan-Rong et al., "Antigen retrieval technique: A novel approach
to immunohistochemistry on routinely processed tissue sections," , in
Gu, J, ed. Analytical Morphology: Theory, Applications and Protocols, BioTechniques
Books, div. of Eaton Publishing, 1997.
Microwave Irradiation of Cryostat Sections
Accelerates and Improves Nitric Oxide Synthase Staining,
Michele Forte and Jiang Gu, Cell Vision, Vol. 1,
No. 1, 1994. posted with permission.
Working Procedures in Microwave Histology,
Vincent R. Klump, Jr., HT (ASCP), Histology Services, East Haven, CT
Microwave Tissue Processing
From Donna Willis, Harris Methodist Hospital, Ft. Worth, Texas
Microwave Oven Quality Control:
Measuring Output
From Donna Willis, Harris Methodist Hospital, Ft. Worth, Texas
Microwave Biopsy Histoprocessing
Procedure III
From Denise Hardy, ARUP Laboratories, Salt Lake City, UT
Accumate ™ RDO
Rapid bone Decalcification Procedure
Fixation in the MicroWave
Microwave Stimulated Fixation
With Preserve™
Microtomy Techniques
VibratomeŽ Operation
Application Note from Energy Beam Sciences
SEM Sample Preparation
Plasma Chemistry: Applications of Barrel
Reactors
Application Note from Energy Beam Sciences
Plasma Chemistry Applied to Electron Microscopy
(EM) Preparation Procedures
Application Note from Energy Beam Sciences
Carbon Coating Thickness
Dr. Norman Charnley, Department of Earth Sciences, University of Oxford
Spurr Epoxy Sections
Diagnostic Electron Microscopy
on Reembedded ("Popped Off") Areas of Large Spurr Epoxy
Sections
P. Anthony di Sant'Agnese and Karen L. De Mesy-Jensen
Large Block Embedding
and "Pop-Off" Technique
for Immunoelectron Microscopy
Karen L. De Mesy-Jensen and by P. Anthony di Sant'Agnese
Plastic Embedded Tissue
Staining Procedures For Plastic Embedded Tissue
Verified at the Applications Laboratory of the Biomedical Division, Sorvall
Microtomes.
Acid Orcein -- Azure-Eosin
Aldehyde Fuchsin, Gomori's
Azan, Modified Heidenhain's
Azure-Eosin, Modified Nocht's
Amyloid Congo Red (Alkaline)
Giemsa
Hematoxylins
Methyl Green -- Pyronin For Plasma Cells
Methyline Blue -- Basic Fuchsin, Lee's
Schiff Stains
Silver Stains
Van Gieson Stain
Oil Red O Stain
Bob Schoonhoven, University of North Carolina
Alkaline Phosphatase
for Osteoblasts
Patsy Ruegg, Metabolic
Bone Disease Laboratory of the University of Colorado Health
Sciences Center
Acid Phosphatase
Glycol Methacrylate: Embedding and Staining
Technovit® 7100
and 8100 Application Information from Heraeus Kulzer Embedding
Protocols
For Normal, Soft Tissue
For Immunohistochemistry
For Materials Samples
Sectioning and Mounting Protocols
Preparing
Silanized Slides
Staining Protocols
Protocols for
Lymphoid Tissue
Hematoxylin and Eosin
Toluidene
Blue
Prussian
Blue
Periodic Acid
Schiff
Giemsa Stain
Acid Phosphatase
(Barka & Anderson)
Alkaline
Phosphatase (Burstone)
Methyl Methacrylate: Embedding and Staining Technovit® 9100 Application
Information from Heraeus Kulzer
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