Molecular Weight Marker

1. Digest 20 µg l DNA (40 µl DNA if at 0.5 µg/µl)
2. Add 50 µl 10X Tracking Dye
3. Bring volume of the digested DNA up to 500 µl with H2O (410 µl if the DNA is 40 µl): If l DNA is digested in our lab, the volume of H2O varys.
4. Mix, heat to 65 C for 5 min, cool quickly on ice, and store at 4 C.
5. Load 5 µl (200 ng per application)

l Digest Marker DNA Size (in base pair)s and Quantity (5 µl application per lane)

PstI	BstII	HindIII
 11,497	8,454	23,130	95 ng DNA
  5,077	7,242	 9,416	38.8
  4,749	6,369	 6,557	27
  4,507	5,686	 4,507	18.5
  2,828	4,822	 2,322	 9.6
  2,559	4,324	 2,027	 8.4
  2,443	2,323	   125
  2,140	1,929
  1,986	1,371
  1,700	1,264
  1,159	  702
  1,093	  224
    795	  224
    514 
    468
    448
    339
    274
    247
    216
    211
    200
    164
    150
     94
     87
     75
     72