Microbiology

Reagents and Stock Solutions

TE Buffer

10 mM Tris, pH 8.0

1mM EDTA

Store 25 °C

Ampicillin

50 mg/mL in water

Sterilize through 0.22 µm filter

Store -20 °C

Ethidium Bromide

0.5 mg/mL in water

light sensitive

Store 25 °C

50X TAE

242 g Tris base

57.1 mL glacial acetic acid

100 mL 0.5 M EDTA (pH 8)

Volume to 1 L

Store 25 °C

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