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THE FOUNDATION OF SUCCESSFUL RT IN SITU PCR
Gerard J. Nuovo
Director, MGN Medical Research Laboratories, Setauket, New York 11733, USA
Received: 07/25/96; Accepted: 10/02/96; On-line 11/01/96
TABLE OF CONTENTS
- 1. Abstract
- 2. Introductory statement
- 3. The key preparatory steps
- 3.1. Fixative
- 3.1.1. Protease digestion
- 3.1.2. Definition of optimal protease digestion
- 3.1.3. Definitions of suboptimal and over-digestion
- 3.1.4. DNase digestion
- 3.1.5. Direct incorporation of the reporter nucleotide
- 4. A one step protocol for RT in situ PCR
- 5. Applications of RT in situ PCR Matrix metalloprotease expression in cancer as a model for RT in situ PCR
- 5.1. MMP and TIMP expression in cervical cancer
- 6. Concluding remarks
- 7. References
- 8. Entire manuscript
Key words: In situ, PCR, reverse transcriptase
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