Solutions for Hartwell's Complete (HC) Media
- 10X HC (7 amino acid dropout solution)
- 10X YNB solution
- Amino acid solutions
- HC plate recipe
- FOA plate recipe
10X HC (7 Dropout Amino Acid Liquid)
2.4 g tyr
3.2 g ile
2.0 g phe
4.0 g glu
8.0 g thr
4.0 g asp
6.0 g val
16.0 g ser
0.8 g arg
in final volume of 4L - Autoclave
10X YNB
58 g YNB (w/o amino acids and w/o ammonium sulfate)
200 g ammonium sulfate
in a final volume of 4L - Autoclave
Amino Acid Solutions (note these are NOT 10X solutions, see below)
URA 1 mg/ml
ADE 1 mg/ml
LYS 10 mg/ml
MET 2 mg/ml
TRP 10 mg/ml
LEU 20 mg/ml
HIS 10 mg/ml
Example Recipe of Complete Plates
20 g of Agar in 609 ml distilled water in a 2L flask
Autoclave 20 minutes
Add the following:
100 ml 20% sterile glucose 100 ml 10X YNB solution 100 ml 10X HC-7aa dropout solution 35 ml URA solution 20 ml ADE solution 12 ml LYS solution 10 ml MET solution 8 ml TRP solution 4 ml LEU solution 2 ml HIS solution
FOA plates
20 g of Agar in 609 ml distilled water in a 2L flask
Autoclave 20 minutes
Add the following:
100 ml 20% sterile glucose 100 ml 10X YNB solution 100 ml 10X HC-7aa dropout solution 35 ml URA solution 20 ml ADE solution 12 ml LYS solution 10 ml MET solution 8 ml TRP solution 4 ml LEU solution 2 ml HIS solution
Let flask cool to 50 deg C, then add 1.0 g of 5-FOA.
