For details on sourcing reagents and preparing the media, download
our SILAC Reagent Protocol.
For details on covalently coupling antibodies to Protein G sepharose,
download our Covalent Coupling
Protocol.
For details on cell fractionation and preparation of whole cell
vs. nuclear and cytoplasmic lysates, download our Cell
Fractionation Protocol.
For details on trypsin digestion of gel slices for MS analysis,
download our In Gel Digestion
Protocol.
Cell staining &
microscopy
For details on working on with transiently transfected and stably
expressed GFP-tagged proteins (including sections on live cell imaging,
FRAP and FRET by acceptor photobleaching and FLIM/FRET), download
our recent Methods in Molecular Biology chapter
here.
Fixation, permeabilization and immunostaining
of cells (contains images and movies)