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| Home > Analysis Tool > BAC Protocols | |
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Manual of BAC Protocols |
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Contents |
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| Introduction | Construction of Large-Insert Bacterial Clone Libraries |
| Experiment 1 | Preparation of High-quality Megabase-size DNA |
| 1.1 |
Preparation of Megabase-size Nuclear DNA from Plants |
| 1.2 | Preparation of Megabase-size DNA from Animals |
| 1.3 | Preparation of Megabase-size DNA from Insects |
| Experiment 2 | Preparation of BAC and BIBAC Cloning Vectors |
| Experiment 3 |
Generation of Large DNA Fragments from Megabase DNA Embedded in LMP Agarose by Partial Digestion for BAC and BIBAC Cloing: Determination of Optimal Partial Digestion Conditions |
| Experiment 4 | BAC and BIBAC Cloning: Partial Digestion of Megabase DNA, Size Selection, Ligation and Transformation |
| Experiment 5 | BAC Analysis |
| Experiment 6 | Large-insert, Ordered Bacterial Clone Library Manipulation |
| Experiment 7 | BAC and BIBAC Library Screening |
| Experiment 8 | BAC End Isolation |
| Appendix A | Preparation of Yeast Chromosome-size DNA and Lambda DNA Ladder |
| Appendix B | Preparation of Competent Cells for Bacterial Transformation by Electroporation |
| Appendix C | Chemicals and Supplies |
| Appendix D | Solutions and Preparation |
| Appendix E | Restriction Site Maps of Widely Used BAC, BIBAC, PBC and PAC Vectors |
| Appendix F | Nucleotide Sequences: pBeloBAC11, pCLD04541 and pBACe3.6 |
| References Cited | |
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| Last Updated Thursday October 03, 2002 | Questions ? webmaster@hbz.tamu.edu |
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2002 hbz laboratory all rights reserved. Norman E. Borlaug Center, College Station, TX77843 |
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