Native Acrylamide Gels (35 ml)
| 3.5% | 5% | 6% | 8% | |
| 30/0.8% acrylamide | 4.1 ml | 5.8 ml | 7 ml | 9.3 ml |
| 10X TBE | 3.5 ml | 3.5 ml | 3.5 ml | 3.5 ml |
| H20 | 27.4 ml | 25.7 ml | 24.5 ml | 22.2 ml |
Degas 1-2 min
Add 0.25 ml 10% ammonium persulfate
Add 30 microliters TEMED
Pour Gel and wait 20-30 minutes before removing gel comb
Run at 180 volts in 1X TBE Buffer
Steve Hahn
Last modified Fri, Oct 16, 1998
