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Biorad Protein Assay: Bradford
Contributor: Suprya Jayadev
Date: July 14, 1993
Standards: 1 mg/ml BSA stock
- dilute 1:10 to get 0.1 mg/ml BSA
Add To get H-2O
20 µl 2 µg/ml 780 µl
40 µl 4 µg/ml 760 µl
60 µl 6 µg/ml 740 µl
80 µl 8 µg/ml 720 µl
100 µl 10 µg/ml 700 µl
120 µl 12 µg/ml 680 µl
Protocol:
1) Add 1, 2, 3, or 4 µl of concentrated unknown, and bring volume
up to 800 µl with water.
2) Add 200 µl concentrated Biorad reagent and incubate at room temperature
for 5 minutes.
3) Assay absorbance at 595 nm.