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Protocols
- Lab Protocols in Developmental Biology (Experiments in Developmental Biology)
A collection of protocols on Sea Urchin, Zebrafish, amphibians (Xenopus & axolotl), chick,and mouse
http://www.swarthmore.edu/NatSci/sgilber1/DB_lab/D...
Added: Wed Mar 04 2009, Hits: 96, Reviews: 0 Write review Cached -
Cell number counting in preimplantation embryos
(P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)
A simple procedure for determining cell number in preimplantation embryos. While the procedure is written for fixed embryos, fixation is optional since the Hoechst 33342 and 4',6-diamidino-2-phenylindole, dihydrochloride (DAPI) penetrate living cells. The fixation step is useful when embryos will be stored for several weeks before determination of cell number
http://www.animal.ufl.edu/hansen/protocols/embryoc...
Added: Tue May 14 2002, Hits: 356, Reviews: 0 Write review Cached -
In Vitro Production of Bovine Embryos
(P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)
This protocol describe procedures for in vitro production [IVP; i.e. in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro culture (IVC)]of embryos
http://www.animal.ufl.edu/hansen/IVF/default.htm
Added: Tue May 14 2002, Hits: 211, Reviews: 0 Write review Cached -
Lineage Analysis Using Retrovirus Vectors
(Cepko Lab, Department of Genetics, Harvard Medical School and Howard Hughes Medical Institute,)
Describe the use of replication-incompetent retroviral vectors for the analysis of lineal relationships in developing vertebrate tissues.
http://axon.med.harvard.edu/~cepko/lineage/
Added: Tue May 14 2002, Hits: 195, Reviews: 0 Write review Cached -
Mini-Chamber for regulating gaseous environment during culture
(P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)
A simple and inexpensive chamber for regulating gaseous environment of small culture plates, such as those used for culture of preimplantation embryos, can be constructed using disposable media-filtration devises such as Corning’s 115-ml system. The following is a description of how to make such a device
http://www.animal.ufl.edu/hansen/protocols/minigas...
Added: Tue May 14 2002, Hits: 144, Reviews: 0 Write review Cached -
TUNEL procedure for preimplantation embryos
(P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)
A protocol for detecting cell death using TUNEL.
http://www.animal.ufl.edu/hansen/protocols/tunel.h...
Added: Tue May 14 2002, Hits: 299, Reviews: 0 Write review Cached