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Protocols
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Assay for Phage Containing the ß- Galactosidase Gene
(Donis Keller Lab)
This assay is used when working with phage vectors carrying the beta-galactosidase gene (often used for immunological screening). If the cloning event disrupts a normally functional copy of the gene in the vector the resulting plaques would appear clear in the assay. If the phages contain a functional beta-galactosidase gene they will form blue rings around their plaques.
http://wheat.pw.usda.gov/~lazo/methods/donis/lambd...
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Bacteriophage Plaque lifts
(Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
Plaque transfer is typically performed using Amersham gridded, nylon, Hybond-N membranes.
http://www.nottingham.ac.uk/~mbzspd/methods/Bacter...
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Calculating Phage Titers
(Goldberg Lab)
http://wheat.pw.usda.gov/~lazo/methods/goldberg/ph...
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Gel Assay to Determine DNA Content of Phage Lysates
(Donis Keller Lab)
A crude lysate gel assay can be performed to roughly quantitate the DNA in lysates. This is often a valuable time saving step to determine if the phage yield is sufficient to warrant continuing the procedure
http://wheat.pw.usda.gov/~lazo/methods/donis/lambd...
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Growth and Purification of 25-100 µg Lambda Clone DNA
(Donis Keller Lab)
http://wheat.pw.usda.gov/~lazo/methods/donis/lambd...
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Helper Phage Preparation
(Vesicle Trafficking)
http://cmgm.stanford.edu/~vesicle/protocols/helper...
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Isolation of Phage DNA from Liquid Lysates and CsCl Block Gradients
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
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Lambda Methods Media and Solutions
(Donis Keller Lab)
http://wheat.pw.usda.gov/~lazo/methods/donis/lambd...
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Liquid Phage Lysates
(Donis Keller Lab)
http://wheat.pw.usda.gov/~lazo/methods/donis/lambd...
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Long-Term Lambda Phage Storage
(Donis Keller Lab)
Store phage in 7% DMS0.
http://wheat.pw.usda.gov/~lazo/methods/donis/lambd...
Added: Tue May 14 2002, Hits: 68, Reviews: 0
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Phage Media Diagram
(Goldberg Lab)
procedures for phage media preparation
http://wheat.pw.usda.gov/~lazo/methods/goldberg/ph...
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Phage Plate Stock Lysates
(Donis Keller Lab)
To prepare phage lysates to be used for small or large scale phage DNA preps. This method usually produces lysates with titers of 2-8x10e10 pfu/ml.
http://wheat.pw.usda.gov/~lazo/methods/donis/lambd...
Added: Tue May 14 2002, Hits: 100, Reviews: 0
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Phage Purification with DE52
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
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Picking Single Phage Plaques
(Donis Keller Lab)
For isolating single bacteriophage lambda plaques to be used for making lysates
http://wheat.pw.usda.gov/~lazo/methods/donis/lambd...
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Preparation of Phage Lysates
(Goldberg Lab)
Very nice and detailed protocol including recipes for media preparation
http://wheat.pw.usda.gov/~lazo/methods/goldberg/ph...
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Streaking Lambda Phages
(Donis Keller Lab)
Phage are streaked onto a medium to obtain an independent isolate prior to preparing a new lysate
http://wheat.pw.usda.gov/~lazo/methods/donis/lambd...
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Titering Lambda Phages
(Donis Keller Lab)
This procedure is used to find the number of viable phage particles in a lysate. The phage lysate is serially diluted and ...
http://wheat.pw.usda.gov/~lazo/methods/donis/lambd...
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Transfer of Phage DNA from Plaques to Nylon Membrane or Nitrocellulose
(Donis Keller Lab)
To transfer phage DNA to nylon membranes so that a phage library can be screened by hybridization
http://wheat.pw.usda.gov/~lazo/methods/donis/lambd...
Added: Tue May 14 2002, Hits: 52, Reviews: 0
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