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Protocols
- Total RNA isolation protocol (Evrogen)
The procedure is suitable for all types of tissues from wide variety of animal species. The method is based on the well-known protocol of Chomczynski and Sacchi (Chomczynski and Sacchi, 1987), except that all steps are performed at neutral pH instead of acidic as was originally suggested.
http://www.evrogen.com/technologies/RNA-isolation....
Added: Fri Feb 06 2009, Hits: 344, Reviews: 0 Write review Cached -
RNA and protein extraction from the same tissue
(Ivan J Delgado)
http://ivaan.com/protocols/139.html
Added: Sun Mar 01 2009, Hits: 386, Reviews: 0 Write review Cached -
DNA/RNA Extraction from the Same Material
(Pat Heslop-Harrison, Molecular Cytogenetics, University of Leicester)
A protocol for extraction of both DNA and RNA from the same material typically plant leaves.
http://www.le.ac.uk/biology/phh4/dnarna.htm
Added: Fri Feb 06 2009, Hits: 275, Reviews: 0 Write review Cached - How to prepare Molecular Biology grade glycogen (Contributed by Roberto Salvi)
Glycogen can conveniently substitute for tRNA as a carrier for nucleic acid precipitation. Although Molecular Biology grade glycogen can be purchased from a number of vendors, the main disadvantage is that it is very expensive (e.g., About 100 dollars/20-40 mg). Here we present a simple and inexpensive protocol to prepare a large amount of glycogen which is suitable for any kind of Molecular Biology application (we routinely use it for RNA work).
Added: Tue May 14 2002, Reviews: 0 Write review -
Single Step RNA Isolation
(Steven Finkbeiner, UCSF)
http://www.gladstone.ucsf.edu/gladstone/files/fink...
Added: Fri Feb 20 2009, Hits: 206, Reviews: 0 Write review