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Protocols
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Southern Transfer to Nylon Membrane in NaOH
(Donis Keller Lab)
The NaOH transfer may be a more efficient transfer method for larger sized fragments. It is the method of choice for pulsed-field gels containing fragments >= 20 kb.
http://wheat.pw.usda.gov/~lazo/methods/donis/south...
Added: Tue May 14 2002, Hits: 220, Reviews: 0
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Southern Transfer to Nylon Membranes
(Donis Keller Lab)
Southern transfer is the classic method used to move DNA (usually in the form of restriction fragments) from agarose gels to a solid support, e. g. nylon membrane. This protocol is designed for transferring DNA for RFLP analysis. The optimum size range of DNA fragments detected this way is 2 - 25 kb.
http://wheat.pw.usda.gov/~lazo/methods/donis/south...
Added: Tue May 14 2002, Hits: 350, Reviews: 0
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Standard Hybridization Technique for DNA
(Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
The standard solution typically used for both pre-hybridisation and hybridisation is based on that given in Maniatis et al., (1982) with both Denhart's solution and heterologous DNA being replaced by heparin (Singh and Jones, 1984).
http://www.nottingham.ac.uk/~mbzspd/methods/Standa...
Added: Sat Apr 10 2004, Hits: 145, Reviews: 0
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Tail Chop Southern Protocol
(Hedrick Lab, UCSD Cancer Center)
http://hedricklab.ucsd.edu/Protocol/TailChop.html
Added: Tue Dec 10 2002, Hits: 131, Reviews: 0
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