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Protocols
- Genomic DNA Isolation from Blood (Bruce A. Roe Lab, University of Oklahoma)
Standard protocol for DNA isolation from blood
http://www.genome.ou.edu/protocol_book/protocol_pa...
Added: Tue May 14 2002, Hits: 3605, Reviews: 0
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DNA extraction from peripheral blood leucocytes
(DNA Laboratory, Medical School, Malta)
A salting out procedure for extracting DNA from white cells giving good yield and good quality DNA
http://geneticslab.topcities.com/DNAextraction.htm
Added: Sat Dec 06 2003, Hits: 788, Reviews: 0
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DNA Preparation from Blood
(Thomas Ried's Lab, NCI)
Extract DNA from whole blood (EDTA, heparin, citrate) using phenol/chloroform method.
http://www.riedlab.nci.nih.gov/publications/2438.2...
Added: Fri Oct 18 2002, Hits: 2926, Reviews: 0
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DNA Preparation from Lymphoblastoid Cells
(Thomas Ried's Lab, NCI)
Organic extraction of DNA from cultured cells.
http://www.riedlab.nci.nih.gov/publications/2440.2...
Added: Fri Oct 18 2002, Hits: 504, Reviews: 0
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Extraction of DNA from Blood for PCR
(Jackson Labs)
Simple method for preparing DNA from blood for PCR without organic extraction
http://www.jax.org/imr/whole_blood.html
Added: Tue May 14 2002, Hits: 2263, Reviews: 0
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Extraction of DNA from the Whole Blood by Silica Gel
(Institute of Gene Biology, RAS)
Simple and inexpensive procedure in which the pure DNA is extracted from the whole blood within 15-20 min.
http://www.ras.ru/biogen/blood.html
Added: Tue May 14 2002, Hits: 579, Reviews: 0
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Cached - Extraction of genomic DNA from whole blood (Laura-Lee Boodram, Department of Life Sciences, The University of the West Indies)
The protocol is simple and fairly rapid. It does not require the use of organic solvents but rather utilizes salt extraction to precipitate contaminating proteins. High quality DNA is obtained suitable for immediate PCR applications. One can obtain approximately 100-200 ug of DNA from 4-8 mL of fresh or frozen whole blood.
Added: Mon May 17 2004, Reviews: 0
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Manual Isolation of Human DNA from Lymphoblasts or Whole Blood
(Donis Keller Lab)
Nucleated blood cells are used to prepare genomic DNA. The cells are first washed, then lysed and the nuclei (left intact) are pelleted in a low speed centrifuge run.
http://wheat.pw.usda.gov/~lazo/methods/donis/dna/d...
Added: Tue May 14 2002, Hits: 687, Reviews: 0
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