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Summary: Flow cytometry (FCM) is a means of measuring certain physical and chemical characteristics of cells or particles as they pass in a fluid stream by a beam of laser light. The term "flow cytometry" derives from the measurement (meter) of single cells (cyto) as they flow past a series of detectors. Flow Sorting extends flow cytometry by using electrical or mechanical means to divert and collect cells with one or more measured characteristics falling within a range or ranges of values set by the user. The major applications of FCM include the analysis of cell cycle, apoptosis, necrosis, multicolor analysis, cell sorting, functional analysis and stem cell analysis, etc.
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Protocols
- FACS Troubleshootings (Protocol Online)
Archives of FACS troubleshootings
http://www.protocol-online.org/biology-forums/flow...
Added: Wed Feb 11 2009, Hits: 258, Reviews: 0 Write review Cached - Flow Cytometry Analysis (Springer Lab, Harvard University)
Flow cytometry employs instrumentation that scans single cells flowing past excitation sources in a liquid medium. The technology can provide rapid, quantitative, multiparameter analyses on single living (or dead) cells based on the measurement of visible and fluorescent light emission. Flow cytometry is a widely used method for characterizing and separating individual cells. This basic protocol focuses on: measure fluorescence intensity produced by fluorescent-labled antibodies and ligands that bind specific cell-associated molecules.
http://www.cbrinstitute.org/labs/springer/protocol...
Added: Wed Aug 14 2002, Hits: 2387, Reviews: 0 Write review Cached -
Advanced Methodologies in Flow Cytometry
(Cytomety Lab, Purdue Univ)
A large collection of methodological paper on cytometry
http://www.cyto.purdue.edu/flowcyt/research/cytote...
Added: Tue May 14 2002, Hits: 1399, Reviews: 0 Write review Cached -
Direct Immunofluorescence Staining
(U of Florida Flow Cytometry Core Laboratory)
This is the method for direct immunofluorescence labeling; that is, the antibodies have the fluorescent dye attached. Direct labeling is simpler and quicker than indirect labeling.
http://www.biotech.ufl.edu/FlowCytometry/direct.ht...
Added: Tue May 14 2002, Hits: 710, Reviews: 0 Write review Cached -
FCM of leukocytes in whole blood
(David Chambers)
http://pingu.salk.edu/flow/protocols/wholeblood.ht...
Added: Tue May 14 2002, Hits: 386, Reviews: 0 Write review Cached -
Flow Cytometry: Indirect Immunofluorescence Staining of Human Platelets
(BD Pharmingen)
Protocol for general use.
http://www.bdbiosciences.com/pharmingen/protocols/...
Added: Sat Jul 27 2002, Hits: 355, Reviews: 0 Write review Cached -
Fluorescein Labeling
(David Chambers)
http://pingu.salk.edu/flow/protocols/flabel.html
Added: Tue May 14 2002, Hits: 382, Reviews: 0 Write review Cached -
Immunofluorescence Staining of Human Cells by Lysed Whole Blood Method
(BD PharMingen)
Standard protocol
http://www.bdbiosciences.com/pharmingen/protocols/...
Added: Wed Aug 28 2002, Hits: 346, Reviews: 0 Write review Cached -
Immunofluorescent Staining for FACS Analysis
(Flow Cytometry Core Facility, PrincetonUniversity)
General immunofluorescence staining protocol using directly and indirectly conjugated antibodies
http://www.molbio1.princeton.edu/facility/flowcyt/...
Added: Sat Feb 21 2009, Hits: 256, Reviews: 0 Write review Cached -
Immunofluorescent Staining of Mouse and Rat Leukocytes
(BD PharMingen)
Standard protocol including detailed procedures and tips
http://www.bdbiosciences.com/pharmingen/protocols/...
Added: Wed Aug 28 2002, Hits: 410, Reviews: 0 Write review Cached -
Phycoerythrin Labeling
(David Chambers)
http://pingu.salk.edu/flow/protocols/pelabel.html
Added: Tue May 14 2002, Hits: 170, Reviews: 0 Write review Cached -
Protein (Antibody) Fluorescent Labeling
(Mario Roederer)
Gives detailed protocol for conjugation of proteins (principally monoclonal antibodies) to fluorescent dyes. The conjugations fall into four basic protocols: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA, and their derivatives); and Type 4 (used for the initial preparation of non-immunoglobulin proteins like Annexin V).
http://www.drmr.com/abcon/
Added: Tue May 14 2002, Hits: 412, Reviews: 0 Write review Cached -
Staining Procedure for Flow Cytometric Detection of Human Cyclins
(BD PharMingen)
This is a standard protocol used at Pharmingen for Quality Control testing of the anti-cyclin antibodies by flow cytometry. It is a good starting point. However, investigators may need to optimize protocols for their own experimental system. It is particularly useful to refer to the published literature regarding protocols typically used for a given type of protein.
http://www.bdbiosciences.com/pharmingen/protocols/...
Added: Wed Aug 28 2002, Hits: 251, Reviews: 0 Write review Cached -
Standard Flow Cytometry (FACS) Protocol
(Jackson Lab)
FACS Analysis Using Peripheral Blood Cells
http://www.jax.org/imr/facs.html
Added: Tue May 14 2002, Hits: 2161, Reviews: 0 Write review Cached -
Yeast Cell Cycle Analysis by Flow Cytometry
(Susan Forsburg)
http://pingu.salk.edu/flow/protocols/ycc.html
Added: Tue May 14 2002, Hits: 214, Reviews: 0 Write review Cached -
Yeast Prep for FACS Analysis
(Gottschling Lab, Fred Hutchinson Cancer Research Center)
http://labs.fhcrc.org/gottschling/Yeast%20Protocol...
Added: Fri Jul 26 2002, Hits: 281, Reviews: 0 Write review Cached
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