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Protocols
- General ES Cell Protocols/Starting Out With ES Cells (Allan Bradley's Lab, Baylor College of Medicine, Houston, Texas)
ES cell thawing and preservation, maintaining.
http://www.imgen.bcm.tmc.edu/molgen/labs/bradley/e...
Added: Sun Jul 28 2002, Hits: 613, Reviews: 0 Write review Cached - Guidelines for Culturing ES and MEF Feeder Cells (Transgenic Research Center, Stanford University)
Routine maintenance of ES cells, culturing feeder cells, cell freezing and thawing.
http://med.stanford.edu/transgenic/esmeflif.html
Added: Wed Jan 28 2009, Hits: 101, Reviews: 0 Write review -
Aggregation of ES Cells and Eight Cell Stage embryos
(Bowtell Lab)
http://grimwade.biochem.unimelb.edu.au/bowtell/cel...
Added: Tue May 14 2002, Hits: 180, Reviews: 0 Write review Cached -
Aggregation of ES cells with mouse morula-stage embryos
(Practical Approach Online, Oxford University Press)
Describes: Preparation of aggregation wells, ES cell preparation, and embryo preparation and aggregation
http://www.oup.co.uk/pdf/pas/13-10-6.pdf
Added: Mon Sep 02 2002, Hits: 185, Reviews: 0 Write review -
b-Galactosidase Color Assay for Cultured Cells
(Allan Bradley's Lab, Baylor College of Medicine, Houston, Texas)
http://www.imgen.bcm.tmc.edu/molgen/labs/bradley/b...
Added: Sun Jul 28 2002, Hits: 138, Reviews: 0 Write review Cached -
Blastocyst Transfer
(Bowtell Lab)
http://grimwade.biochem.unimelb.edu.au/bowtell/cel...
Added: Tue May 14 2002, Hits: 119, Reviews: 0 Write review Cached -
Cell Culture from Whole Mice Embryo
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
Obtain ES cell from mice embryo for culture
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 232, Reviews: 0 Write review Cached -
Cortical Stem Cell Culture Expansion Protocol
(R&D System)
http://www.rndsystems.com/stem_cell_protocol_detai...
Added: Wed Feb 04 2009, Hits: 37, Reviews: 0 Write review Cached -
Culturing Embryonic Stem Cells on a Feeder Cell Layer
(R&D Systems)
A protocol for culturing BG01V embryonic stem cells on a feeder cell layer of irradiated fibroblasts.
http://www.rndsystems.com/stem_cell_protocol_detai...
Added: Fri Mar 20 2009, Hits: 41, Reviews: 0 Write review Cached -
Electroporation of ES Cells and Isolation of H/R Clones
(Bowtell Lab)
http://grimwade.biochem.unimelb.edu.au/bowtell/cel...
Added: Tue May 14 2002, Hits: 135, Reviews: 0 Write review Cached -
ES-cell injection into mouse blastocysts
(Practical Approach Online, Oxford University Press)
Step by step guide for ES cell microinjection, required reagents and equipment.
http://www.oup.co.uk/pdf/pas/13-10-4.pdf
Added: Wed Sep 04 2002, Hits: 134, Reviews: 0 Write review -
Injection of ES cells into morulae
(Practical Approach Online, Oxford University Press)
http://www.oup.co.uk/pdf/pas/13-10-5.pdf
Added: Wed Sep 04 2002, Hits: 87, Reviews: 0 Write review -
Normaski Optics
(Bowtell Lab)
http://grimwade.biochem.unimelb.edu.au/bowtell/cel...
Added: Tue May 14 2002, Hits: 58, Reviews: 0 Write review Cached -
Preparation of RNA from Cultured ES Cells
(Allan Bradley's Lab, Baylor College of Medicine, Houston, Texas)
http://www.imgen.bcm.tmc.edu/molgen/labs/bradley/r...
Added: Sun Jul 28 2002, Hits: 95, Reviews: 0 Write review Cached -
Production of Completely ES Cell-Derived Fetuses by Aggragation with Tetraploid Embryos
(Nagy Lab)
http://www.mshri.on.ca/nagy/Tetraploid/Tetra.htm
Added: Tue May 14 2002, Hits: 68, Reviews: 0 Write review Cached -
Production of ES Cell Chimeras by Aggregation wtih Eight-Cell Stage Embryos
(Nagy Lab)
To produce completely ES cell-derived embryos a clump of ES cells is sandwiched between two tetraploid embryos. For ES cell aggregation chimeras with diploid embryos, one embryo is aggregated with a clump of ES cells. Even in this case, the ES cell internalization is efficient enough to achieve a high level of ES cell contribution.
http://www.mshri.on.ca/nagy/diploid/diploid.htm
Added: Tue May 14 2002, Hits: 70, Reviews: 0 Write review Cached -
Routine Splitting and Freezing of Cells
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 111, Reviews: 0 Write review Cached -
Vasectomy of Male Mice
(Bowtell Lab)
http://grimwade.biochem.unimelb.edu.au/bowtell/cel...
Added: Tue May 14 2002, Hits: 53, Reviews: 0 Write review Cached