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Protocols
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Bacculovirus Production : for Protein Expression
(Mike A. Dyer)
This is a modified version of the pharmingen Baculogold kit
http://axon.med.harvard.edu/~cepko/protocol/mike/B...
Added: Tue May 14 2002, Hits: 425, Reviews: 0
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Cached - Protein Expression (Contributed by Mark's Lab)
Added: Tue May 14 2002, Reviews: 0
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Protein expression in minimal media
(Chen)
Protocol with application notes.
http://www.biochem.ucl.ac.uk/~chen/protocols/prote...
Added: Tue May 14 2002, Hits: 615, Reviews: 0
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Protein Expression in Pichia
(Dario Neri's labs, Institute of Pharmaceutical Sciences ETHZ)
Pichia pastoris is a methylotropic yeast used to express high amounts of protein. Secreted expression is achieved with a cleavalable faktor. To yield high expression strains it is important to select for multiple copies of the insert in the pichia genome.
http://www.pharma.ethz.ch/institute_groups/biomacr...
Added: Sun Mar 01 2009, Hits: 139, Reviews: 0
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Protein refolding
(Chen)
Proteins expressed in E. coli are often found as insoluble aggregates in inclusion body. It may be possible to increase the solubility for some porteins by reducing incubating temperature during protein expression, use of fusion partners such as GST, thioredoxin and maltose-binding protein, co-expression of chaperones etc.
http://www.biochem.ucl.ac.uk/~chen/protocols/refol...
Added: Tue May 14 2002, Hits: 853, Reviews: 1 Read reviews
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