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Protocols
- A Rapid Method to Isolate Plant Genomic DNA (Atle M. Bones, Norwegian University of Science and Technology)
http://boneslab.bio.ntnu.no/quickprepdna.htm
Added: Tue May 14 2002, Hits: 1605, Reviews: 0
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Cached - Genomic DNA extraction
(CIMMYT Applied Molecular Genetics Laboratory)
Genomic DNA extraction from plant material using CTAB buffer, may also apply to other materials.
http://www.cimmyt.org/english/docs/manual/protocol...
Added: Fri Aug 02 2002, Hits: 3689, Reviews: 0
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- Plant DNA Extraction Protocol (Lazo Lab, USDA)
A. thaliana has a very small haploid genome and this makes obtaining DNA somewhat difficult. The most notable problem is that DNA is usually contaminated with polysaccharide which inhibit restriction enzymes as well as other DNA modifying enzymes. This problem is most easily solved by using young plants which have not accumulated as much polysaccharide as older plants. The best results are obtained with plants that are two to three weeks post germinated.
http://wheat.pw.usda.gov/~lazo/methods/lazo/dnaplm...
Added: Sun Mar 22 2009, Hits: 888, Reviews: 0
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Arabidopsis DNA Purification from Nuclei
(Lazo Lab, USDA)
http://wheat.pw.usda.gov/~lazo/methods/lazo/arabdn...
Added: Sun Mar 22 2009, Hits: 90, Reviews: 0
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Cetyltrimethylammonium bromide (CTAB) Plant DNA Extraction
(BioProtocol)
Detailed protocol for extracting DNA from leaves
http://bioprotocol.bio.com/protocolstools/protocol...
Added: Sun Jul 21 2002, Hits: 1354, Reviews: 0
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Cotton DNA Isolation
(Lazo Lab, USDA)
Isolation of Cotton Genomic DNA from Leaf Tissue
http://wheat.pw.usda.gov/~lazo/methods/lazo/cotton...
Added: Sun Mar 22 2009, Hits: 128, Reviews: 0
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DNA and RNA Double Isolation from Plant Leaf
(Pat Heslop-Harrison and Trude Schwarzacher, Department of Biology, University of Leicester)
A protocol for extraction / isolation of both DNA and RNA from the same material typically plant leaf / leaves
http://www.le.ac.uk/biology/phh4/methods/dnarna.ht...
Added: Sat Mar 27 2004, Hits: 570, Reviews: 0
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DNA extraction using the 'sap extractor '
(CIMMYT Applied Molecular Genetics Laboratory)
http://www.cimmyt.org/english/docs/manual/protocol...
Added: Fri Aug 02 2002, Hits: 483, Reviews: 0
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DNA Isolation from Plant Leaf
(Pat Heslop-Harrison and Trude Schwarzacher, Department of Biology, University of Leicester)
CTAB method
http://www.le.ac.uk/biology/phh4/methods/dnaiso.ht...
Added: Sat Mar 27 2004, Hits: 1080, Reviews: 0
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DNA Purification-Modified CTAB Procedure
(Kim Marshall)
DNA extraction from needles
http://dendrome.ucdavis.edu/Protocols/dna_from_nee...
Added: Tue May 14 2002, Hits: 805, Reviews: 0
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Extraction DNA from Plant Materials
(IDT)
General plant DNA isolation protocol. Plant materials are among the most difficult for high quality DNA extractions. The key is to properly prepare the tissues for extraction. In most cases this involves the use of liquid nitrogen flash freezing followed by grinding the frozen tissue with a mortar and pestle.
http://www.idtdna.com/support/technical/TechnicalB...
Added: Sun Mar 22 2009, Hits: 500, Reviews: 0
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Genomic Plant DNA Extraction for Mapping
(Kay Schneitz Lab)
This protocol is quick, reliable and the DNA obtained can be used for PCR-based mapping using SSLP and CAPS markers.
http://www.unizh.ch/botinst/Cyto_Website/schneitzL...
Added: Tue May 14 2002, Hits: 643, Reviews: 0
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Isolation of genomic DNA and total RNA from same total nucleic acid preparation
(Craig Pikaard's Laboratory at Washington University)
this protocol allows you to isolate both genomic DNA and total RNA from the same tissue sample
http://www.biology.wustl.edu/pikaard/PDFs%20and%20...
Added: Tue Mar 03 2009, Hits: 288, Reviews: 0
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Mitochondria DNA Isolation
(Gerard Lazo Lab)
http://wheat.pw.usda.gov/~lazo/methods/lazo/dnaplm...
Added: Fri Apr 09 2004, Hits: 574, Reviews: 0
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Plant Chloroplast DNA Extraction Protocol
(Lazo Lab, USDA)
http://wheat.pw.usda.gov/~lazo/methods/lazo/dnaplc...
Added: Sun Mar 22 2009, Hits: 97, Reviews: 0
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Plant Genomic DNA Extraction using CTAB
(ARC Centre of Excellence for Integrative Legume Research The University of Queensland)
DNA extraction from plant tissue can vary depending on the material used. Essentially
any mechanical means of breaking down the cell wall and membranes to allow access to
nuclear material, without its degradation is required. For this, usually an initial grinding
stage with liquid nitrogen is employed to break down cell wall material and allow access
to DNA while harmful cellular enzymes and chemicals remain inactivated. Once the
tissue has been sufficiently ground, it can then be resuspended in a suitable buffer, such
as CTAB...
http://www.cilr.uq.edu.au/UserImages/File/Plant%20...
Added: Sun Mar 22 2009, Hits: 462, Reviews: 0
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Plant Nuclear DNA Preparation
(Lazo Lab, USDA)
This method can be used to prepare Petunia cv. Rose du Ciel nuclear DNA suitable for restriction enzyme analysis and cloning. Chloroplast DNA contamination will vary depending on the genotype. Greater chloroplast DNA contamination results when this method is used with the "Mitchell" line of Petunia.
http://wheat.pw.usda.gov/~lazo/methods/lazo/dnaplp...
Added: Sun Mar 22 2009, Hits: 66, Reviews: 0
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Plant Nuclei DNA Extraction Protocol
(Lazo Lab, USDA)
http://wheat.pw.usda.gov/~lazo/methods/lazo/dnapln...
Added: Sun Mar 22 2009, Hits: 92, Reviews: 0
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Plant Total DNA Isolation
(Byeong-Ha Lee at the University of Arizona )
describes a method for isolating DNA from plant tissue
http://bioprotocol.bio.com/protocolstools/protocol...
Added: Sun Jul 21 2002, Hits: 1167, Reviews: 0
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Preparation of Megabase-size DNA
(Clemson University Genomics Institute)
To construct large insert DNA libraries in BAC and YAC vectors, methods must be developed to isolate very high molecular weight DNA - megabase-size DNA
http://www.genome.clemson.edu/groups/bac/protocols...
Added: Tue May 14 2002, Hits: 368, Reviews: 0
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Cached - Rapid DNA Isolation from Phyllanthus Amarus and Other Plant Tissues (Protocol Online)
Added: Mon Feb 02 2009, Reviews: 0
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Single leaf DNA prep for Southern blot analysis of transgenic plants in first generation
(Craig Pikaard's Laboratory at Washington University)
http://www.biology.wustl.edu/pikaard/PDFs%20and%20...
Added: Tue Mar 03 2009, Hits: 136, Reviews: 0
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Streamlined DNA Extraction Protocol
(TGERC)
This procedure has been tested with a variety of Populus species, as well as tobacco and Arabidopsis.The resulting DNA is of sufficiently high quality for PCR (including RAPD), restriction digests, and ligation reactions.
http://www.fsl.orst.edu/tgerc/dnaext.htm
Added: Tue May 14 2002, Hits: 591, Reviews: 0
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