Authors
Leiter EH. Coleman DL. Ingram DK. Reynolds MA.
Title
Influence of dietary
carbohydrate on the induction of diabetes in C57BL/KsJ-db/db
diabetes mice.
Source
Journal of Nutrition. 113(1):184-95, 1983 Jan.
Abstract
Genetically diabetic C57BL/KsJ-db/db and normal littermate mice of both sexes
were fed one of nine defined diets from weaning. The objective was to study
dietary carbohydrate interaction with the
diabetogenic genes through isocaloric substitution of protein for
carbohydrate (either sucrose or dextrin starch) at
concentrations of 0, 8, 24, and 60%. In addition, at 60% concentration, the
effect of type of carbohydrate (e.g., glucose, fructose,
sucrose or dextrin starch) on the deterioration of endocrine pancreatic
structure and function was analyzed. The carbohydrate-free
diet produced the greatest survival to 1 year of age and allowed the
expression of an obesity syndrome uncomplicated by severe hyperglycemia or by
extensive necrosis of pancreatic beta cells and islet atrophy. Those diets
containing intermediate levels of carbohydrate (8 or 24% of
sucrose or dextrin), or 60% dextrin starch, in comparison to diets containing
60% refined carbohydrates, extended life span and produced a
more protracted pathogenesis, but were unable to circumvent eventual severe
hyperglycemia and islet destruction. The diets containing 60% glucose,
fructose, or sucrose all led to the rapid induction of diabetes. Thus,
pathogenesis entails an interaction between dietary
carbohydrate, the db gene, and other diabetes-predisposing
genes in the genome.