Authors
Bouhamidi R. Prevost V. Nouvelot A.
Institution
Laboratoire de neurosciences, UMR 6551 du CNRS, universite de Caen, France.
Title
High protection by grape seed proanthocyanidins (GSPC) of polyunsaturated
fatty acids against UV-C induced peroxidation.
Source
Comptes Rendus de l Academie des Sciences - Serie Iii, Sciences de la Vie.
321(1):31-8, 1998 Jan.
Abstract
The antioxidative effects of grape seed proanthocyanidins (GSPC) were studied
in three in-vitro models in which polyunsaturated fatty acids (PUFAs) in
aqueous solution and mice liver or brain microsomes were used as oxidative
substrates, and UV-C irradiation as the pro-oxidant system. Analysis of UV-C
induced lipid peroxidation was carried out by two methods: gas liquid
chromatography of residual PUFAs and release of thiobarbituric acid-reactive
substances (TBARs) measured by TBA reaction. Results indicate that PUFAs are
more radiosensitive when incorporated in single component micelles than in
mixed component micelles or microsomes. In every case, PUFA peroxidation was
inhibited by low concentrations of GSPC (2 mg/L) while epigallocatecin (EGC)
and epigallocatechin gallate (EGCG) monomers, at an equivalent level of
epicatechin, exhibited no efficacy in our experimental conditions. This
latter effect might be explained by a synergistic action of flavan-3-ol
monomers, dimers and oligomers contained in the grape seed extract.