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>Date: Wed, 20 Jan 1999 00:45:35 -0800 (PST)
>From: Doug Skrecky <oberon@vcn.bc.ca>
>To: extropians-digest@extropy.com
>Subject: Fountain of Youth?
>Sender: owner-extropians@extropy.com
>Reply-To: extropians@extropy.com
>
>Authors
> Shirahata S. Kabayama S. Nakano M. Miura T. Kusumoto K. Gotoh M.
> Hayashi H. Otsubo K. Morisawa S. Katakura Y.
>Institution
> Institute of Cellular Regulation Technology, Graduate School of Genetic
> Resources Technology, Kyushu University, Fukuoka, Japan.
> sirahata:grt.kyushu-u.ac.jp
>Title
> Electrolyzed-reduced water scavenges active
> oxygen species and protects DNA from oxidative damage.
>Source
> Biochemical & Biophysical Research Communications. 234(1):269-74, 1997 May
> 8.
>Abstract
> Active oxygen species or free radicals are considered to cause extensive
> oxidative damage to biological macromolecules, which brings about a variety
> of diseases as well as aging. The ideal scavenger for active oxygen should be
> 'active hydrogen'. 'Active hydrogen' can be produced in reduced
> water near the cathode during electrolysis of
> water. Reduced water exhibits high pH, low
> dissolved oxygen (DO), extremely high dissolved molecular hydrogen (DH), and
> extremely negative redox potential (RP) values. Strongly
> electrolyzed-reduced water, as well as
> ascorbic acid, (+)-catechin and tannic acid, completely scavenged O.-2
> produced by the hypoxanthine-xanthine oxidase (HX-XOD) system in sodium
> phosphate buffer (pH 7.0). The superoxide dismutase (SOD)-like activity of
> reduced water is stable at 4 degrees C for over a month and
> was not lost even after neutralization, repeated freezing and melting,
> deflation with sonication, vigorous mixing, boiling, repeated filtration, or
> closed autoclaving, but was lost by opened autoclaving or by closed
> autoclaving in the presence of tungsten trioxide which efficiently adsorbs
> active atomic hydrogen. Water bubbled with hydrogen gas
> exhibited low DO, extremely high DH and extremely low RP values, as does
> reduced water, but it has no SOD-like activity. These
> results suggest that the SOD-like activity of reduced water
> is not due to the dissolved molecular hydrogen but due to the dissolved
> atomic hydrogen (active hydrogen). Although SOD accumulated H2O2 when added
> to the HX-XOD system, reduced water decreased the amount of
> H2O2 produced by XOD. Reduced water, as well as catalase and
> ascorbic acid, could directly scavenge H2O2. Reduce water
> suppresses single-strand breakage of DNA b active oxygen species produced by
> the Cu(II)-catalyzed oxidation of ascorbic acid in a dose-dependent manner,
> suggesting that reduced water can scavenge not only O2.- and
> H2O2, but also 1O2 and .OH.
>
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