From: Patrick Wilken (patrickw@cs.monash.edu.au)
Date: Mon Nov 30 1998 - 14:54:15 MST
Wow. Perhaps in vivo gene therapy actually will work in my lifetime. Double
wow.
I am not sure I am reading this right, but does the technology in principle
allow you to repair any (and all) mutations in an organism?
Thoughts?
best, patrick
=======================================================
From: http://news.bbc.co.uk/hi/english/sci/tech/newsid_224000/224723.stm
AMERICAN RESEARCHERS HAVE TURNED ALBINO MICE CELLS BLACK.
A team from the Jefferson Medical College, Philadelphia, used what they
describe as a "gene repair" technique to genetically change white albino
mice colour-producing cells to black.
Not only did the cells turn black, but the scientists showed for the
first time that such changes are both permanent and can be passed to
offspring.
They hope the work can be developed into a new form of gene therapy - a
type of treatment that can correct the genetic mutations that give rise
to some hereditary diseases.
POINT MUTATION
The mutation in albino mice - which gives them their white fur and pink
eyes - is caused by a mutation in the tyrosinase gene.
The mutation inactivates an enzyme that is supposed to make melanin,
which gives skin and fur its colour.
By correcting this single alteration in the gene, known as a 'point
mutation,' tyrosinase activity was restored and melanin was again
produced, changing the colour of the mice cells.
"Melanin-producing cells become normal, changing albino to black," says
Dr Kyonggeun Yoon, associate professor of dermatology and cutaneous
biology and biochemistry and molecular pharmacology at the Jefferson
Medical College, Thomas Jefferson University, Philadelphia.
"Once the mutation is corrected, it takes five or six days for the
entire biochemical process to begin producing melanin."
GENE REPAIR
The gene-repair technique was actually developed by Dr Yoon and a
colleague, Dr Eric Kmiec, several years ago.
It requires the synthesis of a strand of DNA interspersed with its
chemical cousin RNA. This small locates and grabs hold of the part of
the gene that has the fault and then stimulates the cell's own repair
mechanism.
It scans the DNA looking for any mismatches or two strands of DNA that
do not seem in sync.
When it finds a mismatch, it replaces one of the chemical bases - the
letters in the genetic code - with one that fits better.
Dr Yoon says the gene-repair technique is far from perfected.
"Much more research is needed to improve the design and make this
technology generally applicable. Skin is an ideal organ on which to test
this technology since it is accessible and can be monitored."
Dr Eric Kmiec comments on the research: "To my knowledge this is the
first demonstration of trait reversal in any mammalian system."
The research is published in Nature Biotechnology on 30 November.
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