From: Smigrodzki, Rafal (SmigrodzkiR@msx.upmc.edu)
Date: Sun May 26 2002 - 18:18:16 MDT
Harvey Newstrom [mailto:mail@HarveyNewstrom.com] wrote:
The literal answer is that it doesn't work. The glow-in-the-dark bunny
didn't really glow in the dark.
### If I remember correctly, GFP and related proteins (yellow, orange) are
not supposed to glow on their own - they only fluoresce under UV light. The
purpose of the experiment was to derive animals with cells marked in a way
allowing easy detection and observation while alive, to facilitate the
tracing of cellular lineages and cell migrations in developing embryos. This
previously was done with lipophilic dyes, which quickly diluted, or with
methods involving fixed and stained tissues, like B-Gal. For its purpose
it's a quite successfull product.
Rafal
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