From: CYMM (cymm@trinidad.net)
Date: Mon Sep 04 2000 - 16:36:35 MDT
Doug,
I'm relatively new here... fill me in on the whole story will you....?
cymm
-----Original Message-----
From: Doug Skrecky <oberon@vcn.bc.ca>
To: extropians-digest@extropy.com <extropians-digest@extropy.com>
Date: Monday, September 04, 2000 8:49 AM
Subject: 30'th update on fly longevity experiments
> This is the 30'th update on my fly longevity experiments. In Run #28
> I tested the effect of various food items on fly longevity. I also
> looked at evidence of toxicity of an anti-obesity citrus aurantium
> extract, which contains 15 mg synephrine.
> It was somewhat surprising that synephrine was associated with
> reduced late mortality. I will have to have another look at this in
> future to see if this advantage was a fluke or not. Coconut juice also
> looks worth a second look.
> Early survival was increased notably by propolis by the day 11
> census, but late survival was actually reduced. The propolis extract also
> contained 500 mg of rosehips. If propolis/rosehip antioxidants
> deteriorate in solution, this might account for these effects.
>
> Run #28 Percent Survival on Day
> supplement 3 7 11 17 20 24 31 35 41 46 51 56
> ______________________________________________________
> control 100 89 68 64 61 57 36 14 14 7 0 -
> eggplant skin 100 88 73 64 64 64 52 36 21 15 0 -
> mung bean hulls 100 100 70 60 60 50 30 20 5 0 - -
> kidney bean hulls 100 95 68 53 53 47 16 11 0 - - -
> coconut juice 96 79 71 71 71 67 58 50 42 38 21 4
> propolis 100 mg 100 100 90 76 71 62 19 10 5 0 - -
> synephrine 15 mg 95 90 60 60 55 55 40 40 30 25 20 10
> kudzu root 500 mg 95 89 68 68 63 58 37 21 11 5 0 -
>
> In Run #29 I attempted to stabilize proanthocyanidins with copper
> chelators so that the early survival benefits noted in earlier runs with
> the grape extract leucoselect, and the pine bark extract pynogenol might
> be extended in time.
> Possibly due to the already excellent short-term survival of the
> controls, leucoselect did not offer any short term advantage. The higher
> dosage of pyconogenol did prove to be advantageous, with the usual
> increase in early survival, paired to a decrease in late survival when
> the proanthocyanidins have oxidized.
> Both reservatrol, and rooibos tea can chelate copper. When I added
> either of these to proanthocyanidin extracts there was a minor benefit on
> survival. When used by itself rooibos tea (1 bag/cup water) yielded the
> best overall survival this time around. In future I will have to try
> resveratrol by itself as well.
>
> Run #29 Percent Survival on Day
> supplement 4 8 14 17 21 28 32 38 43 48 53
> _______________________________________________________
> control 94 94 72 72 67 61 33 22 0 - -
> leucoselect 100 mg 96 91 74 70 61 35 35 9 9 5 0
> +resveratrol 50 mg 100 100 60 60 50 45 40 25 20 10 5
> +rooibos 100 89 74 74 68 47 47 26 21 5 0
> pynogenol 200 mg 100 95 68 68 53 42 37 16 11 5 0
> +rooibos 100 83 83 69 66 48 45 24 17 3 0
> pynogenol 300 mg 100 95 95 85 75 45 15 10 5 5 0
> rooibos 100 100 100 92 85 85 69 54 31 23 7
>
> In a continuation of my cryobiology experimentation, Freezer Run #2
> tried to confirm the earlier benefit noted for pycnogenol at a dosage of
> 200 mg.
> Pycnogenol at the 200 mg dosage proved a bust this time, but doubling
> the dose to 400 mg appeared to slow freezing damage. This is just
> interesting enough that yet another experiment will be needed to
> determine if pynogenol feeding really protects flies from freezing
> damage.
> Note: The milk bottles were placed on their side in the freezer.
> Drops of water placed on the milk bottles housing the flies were all
> frozen after 1 hour, so it looks very likely the flies themselves were
> also frozen, and not merely supercooled. The flies had been stored for 6
> days in their respective milk bottles before being placed in the freezer.
> A few flies had already died in this time, before the freezer experiment
> started.
>
> Freezer Run #2 No. of Percent Survival After
> supplement Flies 0 1 12 hours
> control 22 86 59 0
> 20% less water 17 82 29 0
> pycogenol 200 mg 21 95 57 0
> pycogenol 400 mg 24 88 71 0
>
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