[Virology] RE: particle to pfu ratio

[Randhawa, Parmjeet]

I think parvovirus is smaller than 5.2 kb, more like 2-3 kb. Virus
particles could also be quantitated by electron microscopy but that will
not distinguish viable from non-viable virions.

Parmjeet Randhawa, M.D.
Professor of Pathology,
Division of Transplant Pathology,
University of Pittsburgh, Department of Pathology,
E737 UPMC-Montefiore Hospital,
3459 Fifth Ave, Pittsburgh, PA 15213.
Phone: 412 647 7646
Fax: 412 647 5237

-----Original Message-----
From: virology-bounces from oat.bio.indiana.edu
[mailto:virology-bounces from oat.bio.indiana.edu] On Behalf Of Dunowska,
Magda
Sent: Friday, May 09, 2008 7:14 PM
To: virology from oat.bio.indiana.edu; virology from magpie.bio.indiana.edu
Subject: [Virology] RE: particle to pfu ratio

Doria,
If you have a pure virus prep, maybe you could titrate it (to get PFU/mL
value), and then extract viral DNA and estimate the number of viral
particles based on the amount of viral DNA present, knowing that
parvoviruses have a single-stranded DNA genome about 5.2 kbp in size?
Just a thought...

Magda



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Message: 1
Date: Wed, 7 May 2008 12:26:10 -0400
From: "Bowers, Doria" <dbowers from unf.edu>
Subject: [Virology] particle to pfu ratios
To: <virology from magpie.bio.indiana.edu>
Message-ID:
 
<783CE756031AB141B53257D89039DD75075EFFA3 from DIAMOND.unfcsd.unf.edu>
Content-Type: text/plain;       charset="US-ASCII"

We are wondering what the particle:PFU ratio is for porcine parvovirus.



Any ideas how to acquire this statistic?



Doria Bowers











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