[Virology] Re: Adenovirus info. request (2)

[adrish]

Freeze-thawing a virus suspension will result in perturbation of viral
surface proteins needed for infectivity and thus a decrease in viral
titers. The same sort of thing happens when you repeatedly F/T an
enzyme preparation.
A

Shelly Ogiste wrote:
> Hi I was hoping you could help me with a question. How can you "lose
> titer" in a freeze thaw cycle? If I take a liquid and repeatedly freeze
> it and thaw it, how is the concentration going to change?
> 
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> Shelly