Reverse Transcriptase Assay for SNV
Coca-Cola
ccla at mochaalmondfudge.com
Sun Mar 7 14:22:31 EST 1999
Hi,
I am currently trying to quantitate reverse transcriptase activity in
supernatents of cells which produce SNV particles. While I have obtained a
kit from Boerhinger Mannheim for the detection of RT activity, it fails to
work for SNV. I am told that it may be due to SNV RT being a
Manganese-dependent enzyme opposed to a Magnesium-dependent enzyme. While
this may be true, I would like to know if anyone has ever measured RT activity
of SNV.
Thank for any help anyone might have.
Mark
mpandori at netway.com
More information about the Virology
mailing list