Ad2 DBP

[PANGELETTI at bmg.bhs.uab.edu]

Hi Emad.
 Ad DNA binding protein is an essential viral replication protein.  It 
functions by binding cooperatively to the displaced DNA strand 
during replication.  It is known to participate in melting of DNA 
strands and is also thought to effect binding of cellular factors (NFI 
and NFIII) to the origin.  It actually enhances initiation of 
replication through some combination of these activities.

I would suggest searching the databases with the sequence of your 
synthetic peptide (at low stringency) in order to see what cellular 
proteins might cross-react with the Abs.  I have a suspicion that 
DBP may be similar to cellular ssDNA binding proteins.

As far as what domains are involved with ssDNA or host range, 
there are several good papers detailing the X-ray crystal structure:

 Kanellopoulo, 1995  
Crystallization and preliminary X-ray crystallographic studies on 
the adenovirus ssDNA binding protein in complex with ssDNA. 
J Struct Biol 115, 113-116 (1995)

Weber, 1995 
Predicted structure of the adenovirus DNA binding protein. 
Virus Genes 9, 171-175 (1995)

 Tucker, 1994 
Crystal structure of the adenovirus DNA binding protein reveals a 
hook-on model forcooperative DNA binding. 
EMBO J 13, 2994-3002 (1994)

Good luck. 


    
Peter C. Angeletti
Dr. Jeffrey A. Engler's lab
Department of Biochemistry and Molecular Genetics
University of Alabama at Birmingham 
Adenovirus replication and host interactions
Pangeletti at bmg.bhs.uab.edu