Flu virus titers, freeze/thawing

[Ian A. York]

Flu isn't one of the viruses I've worked with, but our lab is going to be 
playing with some for a while.  We received a large volume of frozen 
virus, which we tossed into our -80 oC freezer while we looked dubiously 
at each other.  We need to thaw and aliquot this.  Can anyone tell me:
-how much reduction in titre we can expect from the freeze-thaw?
-how to accurately titrate the stocks, if we need to repeat the titre?
-anything else a novice flu person should know? 

We're just going to be using this as targets in cytotoxic T cell assays,
so we don't need to get fancy with it. 

Thanks.

Ian
-- 
      Ian York   (iayork at panix.com)  <http://www.panix.com/~iayork/>
      "-but as he was a York, I am rather inclined to suppose him a
       very respectable Man." -Jane Austen, The History of England