CMV promoter & human macrophages

[Laurence Tiley]

Hi Beverley.

Yes the CMVie promoter works in human macrophages. Most studies have 
used promyelocytic cell lines to study this, but there are a few studies that 
have used freshly isolated macrophages. Your "someone" may be confusing 
macrophages with monocytes, that contain a repressor of CMVie activity. 
Monocytes can be infected, but need to differentiate before the promoter 
becomes fully active. The repressor binds to the 21bp repeat, so I guess that 
if this site is mutated, you could get a CMV promoter that works in both.

Hope this was useful.

LT