Retroviruses and viral vectors

[Matthew Dubeck]

I have two questions that I hope somebody can help me answer.

1.  Is there any way to remove reverse transcriptase from a retrovirus?  
By this I mean a way which preserves the viability of the virus (i.e. 
without destroying it).

2.  What is involved in the production of a viral vector?  If I have a 
piece of DNA (or RNA in the case of retroviruses) how do I add it to the 
genome of the virus?  What is standard technique?

Thank you for any help you can offer.

Matthew Dubeck
RRRP87C at prodigy.com