enteroviruses

[SOMERS at PICARD.EVMS.EDU]

I am posting a query in reference to current methods used in the identi-
fication of enteroviruses.  We currently isolate enteroviruses by inoculation
of monkey kidney and MRC-5 cultures.  Presumptive enterovirus isolates are
sent to the state lab for serotyping.  Final identification is a long process
and even the initial presumptive isolation in culture is not rapid.  What are 
others doing?  Is there an accepted RT-PCR method that has been compared with
TC isolation?   Any info and  comments welcome.

Ken Somers, Ph.D.
Diagnostic Virology
EVMS/CHKD Regional Diagnostic Virology Lab