GLYCOSYLATION

[MSLADE at RNA.BIO.MQ.EDU.AU]

All these expression systems do not make complex type N-linked oligosaccarides 
and do not produce sialic acids.  Bacculovirus is likely to be most like 
mammalian glycosylation pattern, adding high mannose N-linked oligosaccharides.  
O-linked glycosylation in plants can be very different structures to mammalian 
glycosylation (eg containing xylose) and can be very immunogenic in mammals.

Even mammalian expression systems may not have authenic glycosylation, if you 
know what the glycosylation should be?

Best wishes, Martin Slade
----------------------------------------------------------------------

>To:            virology at net.bio.net
>From:          bLangridge at ccmail.llu.edu (Bill Langridge)
>Subject:       GLYCOSYLATION
>Date:          13 Sep 1995 20:02:21 GMT
>
>Can anyone tell me if I were to make a glycolsylated viral (HIV) 
>protein in plants, baculovirus or Pichia system. Will all these systems 
>make the proper glycosylation pattern identical to the host protein? In 
>which system would I expect the most deviation from the normal 
>glysoylation pattern?
>
>Thanks for your help!
>
>Bill Langridge
>
>
>