Help:electron microscopy of adenovirus

[charles mccarthy]

In <ECS9509070913A at utoronto.ca> sophia.mittas at utoronto.ca (James
Campbell) writes: 
>
>
>I am trying to get clear micrographs of the fine fiber proteins which
extend from adenoviruses.
>
>I am currently using carbon-coated grids and a Philips300 EM and have
found that staining the virus with 
>sodium silicotungstate produces very nice definition of the virus
capsid, however, the fibres are seldom 
>visible.  Also, the few times I do see the fibres, they don't show up
on the micrographs, or when they do 
>they are very blurry.   I  have noticed that when taking pictures, the
carbon film tends to drift.  
>
>If you have any suggestions on how to prevent this drift or know of
any other techniques for adenovirus 
>fiber staining, I would be very grateful. 
>
>Sophia Mittas
>sophia.mittas at utoronto.ca
>
>
>
Sophia

Try gold (Au).

Charlie

Charles P. McCarthy
Healthcare Consulting and 
    Medical Research
Carmichael, CA, USA