HBV inactivation

[Walter Ogston]

Wolf Hanisch  writes:
> 
> I am working on a project to extract HBV antigen from human serum. I 
> would appreciate any information on a reliable safe method for 
> inactivation of HBV.
> 
A review I have from 1984 (Stevens, CE, et al, in "Viral
Hepatitis and Liver Disease", ed GN Vyas et al. p 275) cites the 
following generally available papers: 

Hilleman, MR et al. (1975) Purified and inactivated human
hepatitis B vaccine: progress report. Am. J. Med. Sci.
270:401-404

McAuliffe VJ et al. (1980) Type B hepatitis: a review of current
prospects for a safe and effective vaccine.  Rev. Infect. Dis.
2:470-492

This is not the whole literature, but it should give you a start.
Hilleman was the developer of the original Merck serum-derived vaccine
that was licensed in the US before the recombinant version was
developed, so that article should specify or point to the inactivation
procedure.  Hope this helps.  
----------------------------------------------------------------------
Walter Ogston				ogston at hobbes.kzoo.edu
Department of Biology			Phone: (616)337-7010
Kalamazoo College			Fax:   (616)337-7251
Kalamazoo, MI 49006-3295